Opioid receptors characterization

ZuKin, S.R. Tempel, A. Gardner, E.L. and Zukin, R.S. Interaction of 3H(-)SKF-10,047 with brain sigma opioid receptors Characterization and autoradiographic visualization. Submitted for publication. 

Pan YX, Xu J, Mahurter L, et al Identification and characterization of two new human mu opioid receptor splice variants, hMOR-lO and hMOR-lX. Biochem Biophys Res Commun 301 1057-1061, 2003... 

Kieffer BL, Befort K, Gaveriaux-Ruff C, Hirth CG. The 6-opioid receptor isolation of a cDNA by expression cloning and pharmacological characterization. Proc Natl Acad Sci USA 1992 89 12048-12052. 

Nishi M., Takeshima H., Fukada K., Kato S., Mori K. cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for kappa-subtype selective ligands. FEBS Lett. 330 77, 1993. 

Pan G., Standifer K., Pasternak G. Cloning and functional characterization through antisense mapping of a K3-related opioid receptor. Mol. Pharmacol. 47 1180, 1995. 

Raynor K., Kong H., Chen Y. et al. Pharmacological characterization of the cloned kappa-, delta-, and mu-opioid receptors. Mol. Pharmacol. 45 330, 1993. 

Simon EJ, Gioannini TL. Opioid receptor multiplicity isolation, purification and chemical characterization of binding sites. In Opioids I. Handbook of Experimental Pharmacology. Vol. 104 (Herz A, ed). Springer, Heidelberg, 1993 3-26. 

Raynor K, Kong H, Chen Y, Yasuda K, Yu L, Bell GI, Reisine T. Pharmacological characterization of cloned kappa, delta and mu opioid receptors. Mol Pharmacol 1994 45 330-334. 

Loh H, Smith AP. Molecular characterization of opioid receptors. Ann Rev Pharmacol Toxicol 1990 30 123-147-... 

Reported applications of BSOCOES include studying the polypeptide antigens on lymphocyte cell surfaces (Zarling et al., 1980), crosslinking labeled (3-endorphin to its opioid receptors (Howard et al., 1985), and isolation and characterization of calcitonin receptors in rat kidney (Bouizar et al., 1986). 

Zukin RS, Eghbali M, Olive D, Unterwald EM, Tempel A. (1998). Characterization and visualization of rat and guinea pig brain kappa opioid receptors evidence for kappa 1 and kappa 2 opioid receptors. Proc Nati Acad Sci USA. 85(11) 4061-65. 

Rosei MA, Coccia R, Foppoli C, Blarzino C, Cini C, et al. 2000. Cysteinyldopaenkephalins synthesis, characterization and binding to bovine brain opioid receptors. Biochem Biophys Acta 1478 19. 

Progress in the molecular characterization of opioid receptors has been slower than for other cell-surface receptors and, to date, none has been sequenced or cloned and the second messengers mediating opioid actions are still unknown. The literature in this area has been reviewed in 1990 by Lo and Smith [11], who cite three main problems with the opioid receptor it is difficult to solubilize, there are no simple biochemical assays to test the functional integrity of an isolated receptor extract and there are at least three receptor subtypes (designated as mu, kappa and delta). 

Table 1. Main responses of the two best-characterized opioid receptor subtypes...

Pasternak, G.W., Adler, B.A., Rodriguez, J. Characterization of the opioid receptor binding and animal pharmacology of meptazinot. Postgrad. Med. J. 1985, 61, Suppl. 2, 5-12. 

Fang, L., Knapp, R.J., Horvath, R., Matsunaga, T. O., Haaseth, R. C., Hruby, V. J., Porreca, F., Yamamura, H. I. Characterization of fl-IJnaltrindole binding to delta opioid receptors in mouse brain and mouse vas deferens Evidence for delta opioid receptor heterogeneity, J. Pharmacol. Exp. Ther. 1994, 268, 836-846. 

Kieffer, B.L., Befort, K., Gaveriaux-Ruff, C., Hirth, C.G. The 8 opioid receptor, isolation ofcDNA clone by expression cloning and pharmacological characterization, Proc. Natl. Acad. Sci. USA 1992, 89, 12048-12052. 

Okuda-Ashitaka, E., Tachibana, S., Houtani, T Minami, T Masu, Y., Nishi, M., Takeshima, H., Sugimoto, T., Ito, S. Identification and characterization of an endogenous ligand for opioid receptor homologue ROR-C its involvement in allodynic response to innocuous stimulus, Brain Res. Mol. Brain Res. 1996, 43, 96-104. 

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