Oligosaccharides with periodate

Destruction of the galactosyl residues by oxidation with periodate, acetylation of the free hydroxyl groups of the oligosaccharides, or their removal by P elimination all lead to loss of antifreeze activity. 

Antibodies directed against recombinant erythropoietin have been obtained from rabbits immunized with the hormone and Freunds complete adjuvant [64], Two sets of antibodies are present in the serum of rabbits that have been immunized, Fig. (3C). The results of oxidation of the erythropoietin with periodate, Fig. (25A), reaction of the antibodies with lectins of known carbohydrate specificity, Fig. (25B) and inhibition of the antibodies with the structural monosaccharide residues of the hormone, Fig. (26), have established the types of antibodies to be anti-carbohydrate antibodies and the immunodeterminants for these antibodies are oligosaccharides with the following structure N-acetyl neuraminyl a(2->3)... 

Treatment of periodate-oxidized oligosaccharides with bromine, to form the corresponding polycarboxylic acids, followed by hydrolysis and identification of the resulting acids of low molecular weight has also been used as a tool for structural studies. ... 

Overall, these results demonstrated that the binding of lectins with the prepared carbohydrate microarrays are specific and multiple-analytic characterization can be achieved on the aminooxyacetyl-functionalized slide with good selectivity. Furthermore, periodate oxidation of the immobilized oligosaccharides with NaI04 resulted in the loss of lectin binding. These experiments verify that the fabricated carbohydrate microarray is well suited for the selective identification of carbohydrate-binding proteins. 

The cleavage of polysaccharides by hydrolysis with acid, by oxidation with periodate or chromium trioxide, by base-catalysed jS-elimination, by deamination, and by Weermann and Lossen rearrangements has been ably reviewed. A new approach to selective cleavage of the glycosidic bond to the reducing-end residue of oligosaccharides has involved base-catalysed j8-elimination on a model 5-deoxy-5-nitromaltitol derivative. ... 

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