Oligosaccharides presentation

The ABO substances are complex oligosaccharides present in most cells of the body and in certain secretions. On membranes of red blood cells, the oligosaccharides that determine the specific natures of the ABO substances appear to be mostly present in glycosphin-... 

A detailed study of the 0-linked oligosaccharides present on the surface of normal granulocytes, chronic myelogenous leukemia cells, and acute myelogenous leukemia cells has been completed. Structures were elucidated by f.a.b.-m.s. after permethylation, and methylation analysis before and after specific exo-glycosidase treatments. Some of the components were shown by f.a.b.-m.s. to be poly(N-acetyllactosaminyl) oligosaccharides, for example, 29. 

One of the applications of CE for carbohydrate analysis that has shown increase acceptance in the biotechnology industry is the relative distribution of the N-linked oligossacharides on the conserved asparagines residue on the Fc portion of rMAbs. The advantages of CE over traditional methods include enhanced separation efficiencies, shorter analysis time, and improved robustness. The oligosaccharides present on a monoclonal antibody are asialo-N-linked complex biantennary structures with a core fucose, typical of the structures produced in the Chinese hamster ovary (CHO) cells. The main forms share the fucosylated branched core structure but vary in their terminal galactose occupancy ... 

Raffinose Family of Oligosaccharides Present in Some Food Legumes145... 

Rhodinose (174) is the sugar linked in the antibiotic streptolydigin (177)1,259 and is a component of the oligosaccharides present in the rhodomycin family of anthracycline antibiotics. 1,260... 

Prior to the crystallization of jack bean urease it was assumed by the biochemical community that enzymes had no ordered structure. In 1965 the first crystallographic evidence for the mechanism by which enzymes work when Phillips and his group solved the lysozyme structure [6], Details of the structure indicated how the enzyme could bind the oligosaccharides present in its target, bacterial cell wall peptidoglycans, and could respond to the binding event by changing its structure. 

The high number of negative charges in short oligosaccharides present in marine bacteria from Pseudoalteromonas, Shewanella, Alteromonas and Arenibacter could be important for maintaining the integrity of the outer membrane exposed to a peculiar external surrounding. 

Lang L, Reitman M,Tang J, et al. Lysosomal enzyme phosphorylation. Recognition of a protein-dependent determinant allows specific phosphorylation of oligosaccharides present on lysosomal enzymes./ Biol Chem 259 14663-14671,1984. 

Figure 11.29 Viral receptors. Influenza virus targets cells by binding to sialic acid residues (purple diamonds) located at the termini of oligosaccharides present on cell-surface glycoproteins and glycolipids. These carbohydrates are bound by hemagglutinin (interaction circles), one of the major proteins expressed on the surface of the virus. The other major viral surface protein, neuraminidase, iis an enzyme that cleaves oligosaccharide chains to release the viral particle at a later stage of the viral life cycle.

Vitronectin is a glycoprotein with a mass of 70kDa involved in cell adhesion and spreading. Plasma contains about 200-300 p.g/ml. Vitronectin contains three potential A-glycosylation sites. The structures of the N-linked oligosaccharides present on human plasma vitronectin have been determined [78]. Oligosaccharides were released from the... 

Mellis, S., and Banziger, J. (1983a). Structures of Oligosaccharides Present at the Three Asparagin-linked Glycosilation Sites of Human IgD, J. Biol. Chem. 258 11546-11556. 

A systematic identification scheme, based on paper chromatography and t.l.c., acidic and enzymic hydrolysis, and the reaction of carbohydrates with several location reagents, has been applied to the analysis of oligosaccharides present in the urine and faeces of sick children and in the diets they are fed. The identity and origin of these oligosaccharides is described and their relevance to the diagnosis and treatment of children with suspected disorders of carbohydrate metabolism is discussed. 

Mellis, S.J. Baenziger, J.U. (1983) Structures of the Oligosaccharides Present at the Three Asparagine-linked Glycosylation Sites for Human IgD , Journal of Biological Chemistry, 258, 11546-56... 

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