Oligosaccharides elicitors

Yamaguchi T. Ito Y. Shibuya N.(2000) Oligosaccharide elicitors and their receptors for plant defence responses / / Tr. Glycisci. Glykotech. V. 12. P. 113-120. 

Glucan [antifungal-degrades fungal cell wall releases oligosaccharide elicitor]... 

Cote, F., Ham, K.-S., Hahn, M.G., and Bergman, C.W. (1998). Oligosaccharide elicitors in host-pathogen interactions generation, perception and signal transduction. In Biswas, B.B. and Das, H. (Eds), Subcellular Biochemistry, Vol. 29, Plant-microbe interactions. Plenum Press, New York, pp. 385 31. 

In 1997 Nicolaou et al. published the assembly of a heptasaccharide phytoalexin elicitor (HPE, 16) on a solid support (Schemes 5.2-5.4).29 This oligosaccharide... 

The phytoalexin elicitor (PE) family of /1-D-glucans (Fig. 5) was selected as a target because these oligosaccharides had been synthesized previously in solution48,49 and on the solid support.50,51 It was our intention to investigate not only the application of glycosyl phosphates to automated solid-phase synthesis, but also to compare our method to the previously described reports of PE syntheses. 

Nicolau, K. C., Winssinger, N., Pastor, J., and DeRoose, F. (1997) A general and highly efficient solid phase synthesis of oligosaccharides. Total synthesis of a heptasaccharide phytoalexin elicitor (HPE)../. Am. Chem. Soc. 119,449—450. 

The interdependence of methyl jasmonate with chitin- and chitosan-derived elicitors was studied using plant cell suspension cultures of Taxus canadensis. Induction of the biosynthesis of paditaxel and other taxanes was enhanced when methyl jasmonate and elicitors were added 8 days after culture transfer compared to treatments in which only methyl jasmonate or only elicitor was added. The optimal elicitor concentration using AT-acetylchitohexaose was 0.450 pM, but only in the presence of methyl jasmonate. Little, if any, induction of taxane formation occurred with the oligosaccharide alone. The optimal methyl jasmonate concentration was 200 pM using colloidal chitin or oligosaccharides of chitin and chitosan as elicitors. 

Co-mediation of oligosaccharides and MJ has been demonstrated in the rice system in the induction of phytoalexins [100]. Exogenously applied MJ to elicited cells increased production of momilactone A to levels higher than those elicited with AT-acetylchitoheptaose alone. In suspension-cultured cells of parsley the influence of MJ on elicitation using cell walls of Phytophtora megasperma (Pmg elicitor) and chitosan was demonstrated [101]. These results suggested MJ conditioned the parsley suspension cells in a time-dependent manner to become more responsive to elicitation. 

On the other hand, the impact of ethylene in the composition of headspace gases fed to Taxus sp. cultures was very evident. The positive involvement of ethylene was also noted when cultures were elicited with both dissolved and volatilized methyl jasmonate and with chitin- and chitosan-derived oligosaccharides. The effect of the latter compounds on biosynthesis of ethylene by the plant cell cultures brings us to a better understanding of the interdependence of elicitor and hormone concentrations and of cross-talk signal transduction in plants. 

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