Oligosaccharide synthesis kinetics

Dols M, Remaud-Simeon M, Willemot RM, Demuth B, Jordening HJ, Buchholz K, Monsan P (1999) Kinetic modeling of oligosaccharide synthesis catalyzed by Leuconostoc mesenteroides NRRL B-1299 dextransucrase. Biotechnol Bioeng 63 308-315... 

The fructosyltransferase mined from the commercial preparation Pectinex Ultra SP-L is quite stable towards pH, temperature, and the presence of chemicals. The A aculeatus fructosyltransferase showed a high transferase hydrolase raho that provides it with great potential for oligosaccharide synthesis. The enzyme can be easily immobilized on epoxy-activated supports for better performance. Further studies are required to draw mechanishc conclusions on the nature of the kinetics observed with this fructosyltransferase. 

In oligosaccharide synthesis via transglycosylation the reaction is kinetically controlled, and the extent of oligosaccharide formation depends on the partition ratio of the glycosyl-enzyme intermediate between the transfer and hydrolytic reaction (Scheme 1). 

Oligosaccharide synthesis with tri-chloroacetimidates] general aspects, 290 glycopeptide, 306 glycosyl phosphates, 294, 307, 308 hexasaccharide, 299 inverse procedure, 294 kinetic, thermodynamic effects, 293 Lewis acid catalysis, 289 neighboring group participation,... 

Murata, T., Hattori, T., Amarume, S., Koichi, A. and Usui, T. (2003). Kinetic studies on endo-beta-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligosaccharide beta-glycosides using its transglycosylation activity. European Journal of Biochemistry, 270, 3709-3719. 

The anomeric effect is of prime consideration in synthetic carbohydrate chemistry in fact, some of the most important problems in this area, for example, the synthesis of oligosaccharides and carbohydrate-containing antibiotics, involve an understanding of the reactivity and properties of the anomeric center. In a detailed article concerned with the influence of reactant structure and solvent on glycoside synthesis, Schuerch explores how the anomeric effect of a number of reactants influences the course of kinetically controlled glycosidations. 

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