Oligosaccharide separation

Absorption plots of oligosaccharide separations are reproduced in Figure 1 (A maltodextrin, B glucose syrup), those of mono- and disaccharide separations in Figure 2. 

M15. Masamune, H., and Shinohara, H., Biochemical studies on carbohydrates. CCXXIV. Oligosaccharides separated after acetolysis of the group mucopolysaccharide from pig stomach mucus. Fourth report Gastro-trisaccharide. Tohoku J. Exptl Med. 64, 59-63 (1956). 

Fig. 3-122. Separation of various neutral oligosaccharides. - Separator column CarboPac PA-1 eluent (A) 0.1 mol/L NaOH, (B) 0.1 mol/L NaOH + 0.15 mol/L NaOAc gradient 10 min 100% A isocratically, then linearly to 80% B in 60 min flow rate 1 mL/min detection and injection volume see Fig. 3-105 solute concentrations 1 nmol each (taken from [120]).

Fig. 9.7 Schematic representation of oligosaccharides obtained from pork thyroglobuline. The parts without framed units correspond to 9.12. The other oligosaccharides separated are 9.12 + 5, 9.12 + 5 and 6, 9.12 + 5 + 6 + 7 (9.13), and the sulfated dodecasaccharide (9.12 + 5 + 6 + 7 + 8).

The minor oligosaccharides separated by reverse-phase HPLC (peaks... 

Fig. 4.6.2. Chromatograms of oligosaccharides, (a) Oligosaccharides synthesised by the action of E. coli ML 30 on maltose. Separation on Bio-Gel P-2 in pure water at 65°C Gel bed 1.5x127 cm flow rate 28 cm h. 1-11 glucose to maltoundecaose (after John et al. [16]). (b) Malto-oligosaccharides separated on Bio-Gel P-2 in pure water at 55°C Gel bed 2.65x200 cm flow rate 15-32 cm h". 1. glucose 2. maltose etc. (after Kainuma et al. [138]).

HPLC analysis of oligosaccharides from animal tissues or body fluids has been used to determine their molecular weight distribution. The majority of complex oligosaccharide separations has been carried out using chemically bonded amine columns which separate molecules on the basis of chain length, although reversed phase HPLC has been used to separate human milk oligosaccharides (Dua and Bush, 1983). 

Horowitz ST, Roseman S, Blumental HJ (1957) The preparation of glucosamine oligosaccharides separation. J Am Chem Soc 79 5046... 

Nobre C, Suvarov P, De Weireld G. Evaluation of commercial resins for fructo-oligosaccharide separation. New Biotechnol 2014 31(1) 55—63. 

Vente JA, Bosch H, de Haan AB, Bussmann PJT. Comparison of sorption isotherms of mono- and disaccharides relevant to oligosaccharide separations for Na, K, and Ca loaded cation exchange resins. 

Figure 3.238 Separation of kestose and related oligosaccharides. Separator column CarboPac PA1 eluent 0.1 mol/L NaOH + 0.02 mol/L NaOAc flow rate 1 mL/min detection ...

Table 3.31 Chemical structures of oligosaccharides separated in Figure 3.255.

Next to MALDI-MS, the use of ESt-MS is important in oligosaccharide and glycan characterization, especially because it can be combined with on-line LC separation of complex mixtures. The most important LC methods in glycan and oligosaccharide separation are HILIC [184], HPAEC, and, after derivatization by reductive amination with 2-aminobenzamide or 2-aminoacridone, RPLC on either Cjg or porous graphitized carbon materials [185]. 

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