Disaccharides separation

Absorption plots of oUgosaccharide separations are reproduced in Figure 1 (A maltodextrin, B glucose syrup), those of mono- and disaccharide separations in Figure 2. 

Fig. 3-110. Gradient elution of various mono- and disaccharides. - Separator column IonPac AS6A eluent (A) water, (B) 0.05 mol/L NaOH gradient linear, from 7% B to 100% B in 15 min flow rate 0.8 mL/min detection pulsed amperometry at a Au working electrode with post-column addition of NaOH injection volume 50 pL solute concentrations 15 ppm inositol (1), 40 ppm sorbitol (2), 25 ppm fucose (3) and deoxyribose (4), 20 ppm deoxyglucose (5), 25 ppm arabinose (6), rhamnose (7), galactose (8), glucose (9), xylose (10), mannose (11), fructose (12), melibiose (13), isomaltose (14), gentiobiose (15), and cellubiose (16), 50 ppm turanose (17), and maltose (18).

Figure 3.215 Isocratic separation of sugar alcohols, anhydrosugars, and mono- and disaccharides. Separator column Metrosep Carb 2 column dimensions 150 mm x 4 mm i.d. column temperature 45 °C eluent lOmmol/L NaOH flow rate 1 mL/min detection pulsed amperometry on a gold working electrode Injection volume lOOpL peaks 0.64 mg/L...

Figure 3.235 Gradient elution of various disaccharides. Separator column CarboPac PA1 eluent (A) water, (B) 0.05 mol/L NaOH H-1.5 mmol/L acetic acid gradient 20% B for iso-cratically for 5 min, then linearly to 100% B in 15 min flow rate 1 mlVmin detection pulsed...

Scheme 5.—Typical Cleavage of Heparin (Arbitrary Sequence) with Heparinase (HEPase) and Heparanase (HSase). [Fragments longer than a tetrasaccharide may be obtained by separate use of each enzyme. The combined use of the two enzymes produces only disaccharides.]...

The hydrolysis products of methylated disaccharides may be separated by p-phenylazobenzoylation followed by chromatographic adsorption, or the methanolysis products of the methylated disaccharides may be separated due to differential water solubilities of the p-phenylazo-benzoates.110... 

Various fully-acetylated monosaccharides and disaccharides or sugar alcohols are readily separable by chromatographic adsorption on Mag-nesol (a hydrated magnesium acid silicate)111 and the unacetylated substances may be separated on clay columns.112... 

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