Oligosaccharides chromatographic separation

Fig. 1 (A) Chromatographic separation of sugars. Track 1 fructose, 2 sucrose, 3 glucose, 4 mixture of the substances in tracks 1-3, 5 mixture of substances in tracks 1-3 and 6, 6 Fructo-oligosaccharides, 7 1-kestose, 8 mixture of glucose, maltose, maltotriose and maltotetraose. (B) Absorption scan of track 5 with 200 ng each substance per chromatogram zone 1 = fructosyl-nystose, 2 = nystose, 3 = 1-kestose, 4 = fructose, 5 = sucrose, 6 = glucose.

Yoshinaka, Y., Ueda, Y., and Suzuki, S. Ion-pair chromatographic separation of glycoprotein-derived oligosaccharides as their 8-aminopyrene-l,3,6-trisulfonic acid derivatives./. Chromatogr. A. 2007, 1143,83-87. 

A general essay on column chromatography of carbohydrates was written by Capek and Stanek [123], the same authors [224] reviewed polysaccharides, and Juficova and Deyl [225] summarized chromatographic separation of polysaccharide-protein complexes. Reviews specialized on ion exchange chromatography of oligosaccharides... 

Reversed phase HPLC is only rarely used for the chromatographic resolution of saccharides however, in instances where only occasional separations are required the wide availability of reversed phase columns in many laboratories saves the purchase of a specialised carbohydrate column. Reversed phase HPLC has been used primarily for the separation of oligosaccharides and separation is dependent on the degree of polymerisation (Cheetham et al., 1981). Organic phase modifiers such as -alkylamines can be used to provide increased capacity and selectivity for saccharides (Lochmuller and Hill, 1983). 

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