Oligonucleotides defined

This specific base-pairing capability of oligonucleotides defines the structure of... 

Oligonucleotide A short, defined sequence of nucleotides joined together in the typical phosphodi-ester linkage. 

The second NIST human DNA SRM is a PCR-based DNA Profiling Standard. The PCR was first described by Saiki et al. (1985,1989). Since then it has developed into a highly versatile and widely used detection, identification, manipulation and analysis tool in molecular biology, including DNA profiling. In brief, two short synthetic oligonucleotides, or primers, are used to define an intervening DNA sequence... 

Synthetic oligonucleotides are very important tools in the study and manipulation of DNA, including such techniques as site-directed mutagenesis and DNA amplification by the polymerase chain reaction. The techniques for chemical synthesis of oligonucleotides are highly developed. Very efficient automated methodologies based on solid phase synthesis are used extensively in fields that depend on the availability of defined DNA sequences.52... 

D Ham, C. Romieu, A. Jaquinod, M. Gasparutto, D. Cadet, J. Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5- hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins Kinetic and mechanistic aspects. Biochemistry 1999, 38, 3335-3344. 

Primeran oligonucleotide or pair of oligonucleotides used to direct an activity to a region of nucleic acid. With PCR (polymerase chain reaction), a pair of primers defines the area of the genome to be amplified. 

After this brief introduction, the chapter will focus on solvolysis reactions and acid-base equilibria of various platinum compounds and on species distribution of isomeric [PtCl2(NH3)2] in aqueous solution in Section 2. Binding of platinum compounds to monomeric nucleobase derivatives will be discussed in Section 3, while Section 4 pays attention to the reactions of Pt-nucleobase complexes with different nucleophiles. And finally, the interactions of Pt with DNA and defined oligonucleotides will be discussed in Section 5. 

Bioaffinity chromatography, 6 399—400 Bioantimutagen, vanillin as, 25 556 Bioassay dyes, 9 518 Bioassays, microfluidics in, 26 968-969 Bioaugmentation, defined, 3 758t Bioaugmentation/bioremediation effluent treatment, 9 436, 438 Bioavailability, of antisense oligonucleotides, 17 628 Biocatalysis, 3 668-683 16 395. See also Biocatalyst entries... 

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