Proteins kinetics

HS Chan, KA Dill. Protein folding m the landscape perspective Chevron plots and non-AiT-henius kinetics. Proteins 30 2-33, 1998. 

Chan, H. S., and Dill, K. A. (1998). Protein folding in the landscape perspective Chevron plots and non-Arrhenius kinetics. Proteins Struct. Fund. Genet. 30, 2-33. 

Proteins in vivo protein-protein interactions, protein folding kinetics, protein subunit exchange, enzyme activity assay, etc. 

ACTIN ASSEMBLY KINETICS MICROTUBULE ASSEMBLY KINETICS PROTEIN POLYMERIZATION KINETICS NUCLEIC ACID RENATURATION KINETICS Nucleic acid structure,... 

These methods work best at nanomolar chemical concentrations so that the focal volume contains typically 1 to 100 molecules on average. Because the method is so sensitive, it is susceptible to perturbation by background fluorescence and instrumentation fluctuations. These problems have become quite tractable during the last decade, such that FCS now supports more than 100 publications per year. A current challenging application is analysis of protein folding kinetics, protein structure fluctuations, and ultrafast chemical kinetics by new methods yet to be published. 

Sy SK, Ciaccia A, Li W, Roberts EA, Okey A, Kalow W, Tang BK. Modeling of human hepatic CYP3A4 enzyme kinetics, protein, and mRNA indicates deviation from log-normal distribution in CYP3A4 gene expression. Eur J Clin Pharmacol 2002 58 357-365. 

Time-Resolved, Structural Analysis-Correlation of Reaction Kinetics-Protein Conformations and Evolution of Reaction Intermediates... 

Sampling Kinetic Protein Folding Pathways using All-Atom Models... 

P.G. Bolhuis Sampling Kinetic Protein Folding Pathways using All-Atom Models, Lect. Notes Phys. 703, 393-433 (2006)... 

J. D. Bryngelson, J. N. Onuchic, N. D. Socci et al. Funnels, pathways, and the energy landscape of protein-folding - a synthesis. Proteins, 21 (1995), 167 D. K. Klimov and D. Thirumalai. Criterion that determines the foldability of proteins. Physical Review Letters, 76 (1996), 4070 H. S. Chan and K. A. Dill. Protein folding in the landscape perspective chevron plots and non-arrhenius kinetics. Proteins, 30 (1998), 2. 

Chan H, Dill K (1998) Protein folding in the landscape perspective chevron plots and non-arrhenius kinetics. Proteins Struct Funct Genet 30 2-33. 

The term lubricant of life was perhaps first used (in the sense being used here) by Barron et al. [24]. These authors observed by Raman spectroscopy fast dynamic events (in the picosecond timescale) where individual amino acid residues flicker between different secondary structure states. This flickering was attributed to fast dynamic events in bulk water and mediated through HBs of the amino acid residues with surrounding water molecules. These events driven by water could be the guiding force in many functions of proteins (folding and unfolding, enzyme kinetics, protein-DNA interaction), and hence water was termed the lubricant of life. 

The discussion of the biological functions of water details not only the stabilizing effect of water in proteins and DNA, but also the direct role that water molecules themselves play in biochemical processes, such as enzyme kinetics, protein synthesis, and dmg-DNA interaction. The overview of the behavior of water in chemical systems discusses hydrophilic, hydrophobic, and amphiphilic effects, as well as the interactions of water with micelles, reverse micelles, microemulsions, and carbon nanotubes. 

Kim SM, Bums MA, Hasselbrink EF (2006) Electro-kinetic protein preconcentration using a simple glass/ poly(dimethylsiloxane) microfluidic chip. Anal Chem 78(14) 4779 785... 

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