Oligonucleotide arrays, photolithographically synthesized

Forman, J.E., et al. Thermodynamics of duplex formation and mismatch discrimination on photolithographically synthesized oligonucleotide arrays. ACS Symposium Series, 1998, 682, p. 206-228. 

Thermodynamics of Duplex Formation and Mismatch Discrimination on Photolithographically Synthesized Oligonucleotide Arrays... 

Micro-arrays can be manufactured in two different ways, by synthesis in situ or by delivery. In the in situ synthesis approach, nucleic acids are synthesized directly on a chip surface. This is made possible by a light-directed chemical synthesis technology developed by scientists at Affymetrix. By using different sets of photolithographic masks, scientitsts can define the chip exposure sites and, thereby, the sequence of the oligonucleotides. In this way, arrays can be synthesized with up to 400,000 different oligonucleotides in an area of 1.6 cm. Every spot contains about... 

Probe arrays and DNA target. The studies described here have been carried out with arrays of 10-, 12-, 14-, 16-, 18-, and 20-base oligonucleotide probe synthesis sites 200 jim X 200 jim in size. The probes are covalently attached to a siloxane derivatized 15 mm X 15 mm x 0.7 mm borosilicate glass substrate and were synthesized using photolithographic techniques that have been described elsewhere. U... 

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