Nucleic Acids and Related Compounds

There are several lines of experimental evidence to support the Watson-Crick structure (Mahler and Cordes, 1966). We are concerned here only with the type of evidence obtainable from infrared spectroscopy, an example of which is the following Thin sheets of sodium or lithium DNA, equilibrated with D2O, show charactmstic bands at 1685, 1665, and 1644 cm (weak bands at 1620 and 1575 cm ) that have been assigned to C=0, C=N, and C=C stretching and NHj deformation vibrations of the bases in the helical pattern. The band intensity at 1685 cm of DNA in DjO is reduced significantly, while those at 1665 and 1620 cm are increased when the molecular structure is denatured by heat, alkali, formamide, or deoxyribonuclease [see below] (Bradbury et al., 1961 Kyogoku et al, 1961). 

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Spectra, Infrared, of Nucleic Acids and Related Compounds (Shimanouchi, Tsuboi, and Kyogoku)... 

V. G. Shore and A. B. Pardee, Fluorescence of some proteins, nucleic acids and related compounds, Arch. Biochem. Biophys. 60, 100-107 (1956). 

C Chemical Shifts of Nucleic Acids and Related Compounds... 

The principles of attachment of molecules to polysaccharides with concomitant insolubilization, discussed in the preceding two Sections, also apply to nucleic acids. The insolubilization of nucleic acids and polynucleotides provides materials useful (a) for fractionation and purification of other nucleic acids and related compounds,(b) for multiplication and isolation of single nucleic acid strands by base-pairing, " (c) for base-sequence determination, (d) as afiR-nants, " templates, and substrates for nucleic acid-related enzymes, and (e) as aflBnants for nucleic acid-binding proteins. ... 

Generally, nucleic acids and related compounds are not described as alkaloids. However, in this volume because the definition of alkaloids is expanded according to recent trends, these compounds are also considered. 

Hetero-calixarenes represent another interesting group of calixarene-related compounds that can be used for the interaction with nucleic acids and related compounds. Due to their constitutional variation their structural features significantly differ from the corresponding original calixarene scaffolds. Still there is a number of examples worth considering in the broad context of calixarenes and nucleic acids. 

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