Nuclear scintillators

ABSTRACT. Normal human, hemoglobin free erythrocyte ghosts were labelled with C-N-Ethylmaleimide and incubated at two different temperatures 4 C and 37 C. Gel electrophoresis, autoradiography and nuclear scintillation counting techniques were used in analysing which proteins were labelled. 

In this research, NEM binding sites to normal human erytij cyte membranes have been investigated by labelling the membranes with C-NEM. Gel electrophoresis, autoradiography and nuclear scintillation counting tecniques have been used to show which protein components were labelled. 

Results of nuclear scintillation counting for samples incubated at 4 C are shown in Figure 5. It was observed that 35 % of the total NEM was bound to spectrin and 50 % of the NEM was bound to spectrin-actin complex. 

E. Rapkin, Picker Nuclear Scintillator, 11 No. 1 L. Picker Nuclear, White Plains. New York, 1966... 

The concrete block walls of the cell housing the generator tube and associated components are 1.7 meters thick. The facility also includes a Kaman Nuclear dual-axis rotator assembly for simultaneous transfer and irradiation of reference and unknown sample, and a dual Na iodide (Nal) scintillation detector system designed for simultaneous counting of activated samples. Automatic transfer of samples between load station to the rotator assembly in front of the target, and back to the count station, is accomplished pneumatically by means of two 1.2cm (i.d.) polyethylene tubes which loop down at both ends of the system and pass underneath the concrete shielding thru a pipe duct. Total one-way traverse distance for the samples is approx 9 meters. In performing quantitative analysis for a particular element by neutron activation, the usual approach is to compare the count rates of an unknown sample with that of a reference standard of known compn irradiated under identical conditions... 

NUCLEAR COUNTING OF TAGGED EXPLOSIVES BY LIQUID SCINTILLATION... 

E. Rapkin, Guide to Preparation of Samples for Liquid Scintillation Counting , New England Nuclear Corp, Boston, Mass (1975)... 

Uniformly labeled C-8-D with a specific activity of 2.99 juc/mg was administered orally to pregnant females at 2 /xg/kg/day from 6-15 days of gestation. Three females were sacrificed on alternate days during days 6-20 of pregnancy. Triplicate samples of fetus, placenta, blood, brain, abdominal fat, and sartorius muscle were procured from each female. The samples were dissolved in 1 ml of Soluene (Packard Instruments) to which 15 ml of Aquasol were added. Each sample vial was counted for 30 min in a Nuclear Chicago Mark I liquid scintillation counter. 

Radioactivity Analysis. Samples of urine, feces, and tissues were combusted to COo and analyzed for radioactivity (5). By using this method the recovery of radioactivity from samples spiked with C was 95 dt 5%. To determine the radioactivity expired as CO2, 5-ml aliquots of the solution used to trap the CO2 were added to 15 ml of a scintillation counting solution containing 4 grams 2,5-diphenyloxazole (PPO) and 0.1 grams l,4-bis-2(5-phenyloxazolyl)-benzene (POPOP) per liter of 1 1 toluene 2-methoxyethanol. Samples were counted for radioactivity in a Nuclear Chicago Mark II liquid scintillation counter. Counting eflSciency was corrected by the internal standard technique. 

Commonly, in vitro determination of HDAC activity is a manual assay utilizing a coupled two-step process, including enzymatic deacetylation of a substrate followed by reaction termination and readout [10]. Assays utilize nuclear extracts and substrates containing labeled (radioactive or fluorescent) acetylated histones. For the isotope-based assays, the enzymes are incubated with acetate-radiolabled histones prepared from chicken reticulocytes or chemically [ Hjacetylated peptide substrates, and the enzymatic activity is determined by liquid scintillation counting [11]. Alternatively, histones may be obtained from cells following treatment with [ H]acetyl-CoA [12]. The caveats of these approaches include the variability of prelabeled acetylated core histones within preparations, potential high costs, their labor-intensive nature and the presence of radioactive waste. 

Neon is also used in scintillation counters, neutron fission counters, proportional counters, and ionization chambers for detection of charged particles. Its mixtures with bromine vapors or chlorine are used in Geiger tubes for counting nuclear particles. Helium-neon mixture is used in gas lasers. Some other applications of neon are in antifog devices, electrical current detectors, and lightning arrestors. The gas is also used in welding and preparative reactions. In preparative reactions it provides an inert atmosphere to shield the reaction from air contact. 

The examples for radioactive labeling by phosphorus-32 ( P) and iodine-125 ( 1) in this chapter were chosen for two reasons on one hand, they are relatively easy to do, and on the other hand, the measurement of radioactivity is simple. is counted in water in a liquid scintillation counter by measuring the Cerenkov radiation and is measured in a gamma counter. Both isotopes may be detected also by autoradiography. A further advantage of both isotopes is their short half-life, which eases the disposal of nuclear waste. 

An expensive method is the use of nuclear radiation to obtain information on the level in an apparatus. The nuclear sensor is mounted at one side, and at the other side a scintillation counter is fixed near the surface of the apparatus. Both systems are sheathed with lead-screen shields to give protection from nuclear radiation. A continous level indicator using nuclear radiation is very complicated and therfeore seldom applied. 

They also carried out end group analysis by means of a Chicago Nuclear liquid scintillation counter to count C14-labeled end groups. 

Scintillation detectors -m nuclear medical imaging [MEDICAL IMAGING TECITNOLOGY] (Vol 16) -use m x-ray spectroscopy [SPECTROSCOPY, OPTICAL] (Vol 22)... 

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