Nuclear features

Bouin s solution is one of the traditional ways to harden cell pellet. Some cytologists believe it provides the best cellular details, especially nuclear features in cell blocks.28 The major steps are (1) After centrifugation, fix the cell pellet with Bouin s solution. (2) After 2h, discard the solution. (3) Remove the hardened cell pellet from the tube, wrap it with lens paper, and transfer it into a cassette for further processing. We have been using this method for many years. In our experience, most of the time, ICC results are consistent with IHC from the surgical specimen. The biggest drawback of this method is the toxicity of Bouin s fixative which creates biohazard and safety issues for the laboratory. We also found cell blocks gave poor fluorescence in situ hybridization (FISH) results after Bouin s fixation. 

While neuron-specific enolase (NSE) has been described as a marker of neuronal tumors in reviews and texts, this author finds that it has better uses than this. The shortcoming for neuronal tumors is its propensity to also stain glial tumors, which commonly need to be distinguished from neuronal tumors. 7 Experimental IFIC stains such as Neu-N identify neurons by showing their on-target nuclear features rather than their cytoplasmic or confusing surface features. " 425 cases refractory to immu-nohistochemical stains, electron microscopy positively identifies Nissl substance, neurofilaments, neurosecretory granules, and synapses in neoplastic cells. 

Reactive astrocytosis is associated with many brain tumors. Nuclear features of GFAP-positive cells must be examined to distinguish reactive from neoplastic... 

Lesion Interrelationship of GFAP+ Cells Intracellular Distribution of GFAP Nuclear Features Other Tissue Elements... 

Papillary thyroid carcinoma is a tumor of thyroid follicular epithelium with papillary and follicular growth pattern and the characteristic nuclear features. Papillary thyroid carcinoma makes about 70% of all thyroid carcinomas, often associated with radiation exposure or high iodine intake, and mainly metastasizes by invasion of lymph vessels. Papillary thyroid carcinoma frequently associated with different rearrangements mainly involving the RET/PTC gene discussed in a previous section. 

Follicular thyroid carcinoma is also a tumor of thyroid follicular epithelium exhibiting pure follicular differentiation and lacking the nuclear features charac-... 

In this calculation we confine our attention to a study of the nuclear features of the proposed system. 

Boranes, boron clusters, and in particular, carboranes are of special interest due to their unique properties that cannot be found in organic counterparts. These uniqne properties are based either on the element boron, due to its electron deficiency, or on the structnral featnre of the cluster compound. Borane clusters as a class of materials have a wide range of potential applications. This is not only due to their unique electronic and nuclear features the fields of application, to name but a few, range from materials science through medical applications to catalysis, which will be described in more detail below [13]. Carboranes can be applied as liquid crystals in electro-optical displays [14], non-linear optics [15], and ion-selective electrodes [16] in the materials science arena. If carboranes are vaporized and fired at high temperatures they create boron films that are applied in Tokamak reactors for nuclear fusion [17]. Boranes have furthermore found application in airbag propellant systems in cars [18], as the stationary phase in gas chromatography [19] and in metal ion extraction systems, for example, for nuclear waste [20]. In medical applications, boron neutron capture therapy (BNCT), a special field of anti-cancer therapy, is noteworthy. 

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