Nonradioactive in-situ hybridization

Hopman, A.H.N., Wiegant, J., Tesser, G.I., and Van Duijn, R (1986) A nonradioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten hgands. Nucleic Acids Res. 14, 6471-6488. 

Matsunaga S, Kawano S, Higashiyama T, InadaN, Kuroiwa T. Clear visualization of the products of nonradioactive in situ hybridization in plant tissue by simple dark-field microscopy. Micron 1997 28 185-187. 

In the tissue sections, a hybridization is performed with a highly specific probe in order to detect the target (either amplified by indirect in situ RT-PCR or unamplified). For the preparation of nonradioactive digoxigenin-labeled probes, one should refer to the guidelines of the company manuals Genius M System User s guide for membrane hybridization and Nonradioactive in situ hybridization application manual (2nd ed., Boehringer Mannheim). 

Nonradioactive in Situ Hybridization Using Alkaline Phosphatase-Labelled Oligonucleotides S. J. Augood, E. M. McGowan, B. R. Finsen,... 

Combining Nonradioactive in Situ Hybridization with Immunohistological and Anatomical Techniques Petra Wahle... 

Nonradioactive in Situ Hybridization Simplified Procedures for Use in Whole Mounts of Mouse and Chick Embryos... 

Schmitt A, Asan E, Puschel B, Kugler P (1997) Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats nonradioactive in situ hybridization and comparative immunocytochemistry. J Neurosci 77 1-10. 

Esclapez M, Tillakaratne NJK, Tobin AJ, Houser CR (1993) Comparative localization of mRNAs encoding two forms of glutamic acid decarboxylase with nonradioactive in situ hybridization methods. J. Comp. Neurol, 331, 339-362. 

The NBT-BCIP is probably the most stable chromogen-substrate system, and does not form precipitates in the developing solution for several hours. It is well suited for overnight development in nonradioactive in situ hybridization also. 

Tenberge KB, Stellamanns P, Plenz G, Robenek H. Nonradioactive in situ hybridization for detection of hydrophobin mRNA in the phytopathogenic fungus Claviceps purpurea during infection of rye. Eur J Cell Biol 75 265-272, 1998. 

Nonradioactive in Situ Hybridization Application Manual, Boehringer Mannheim GmbH Biochemica, 1992. A copy of this 75 page booklet may be obtained by writing to PO box 310120, D-6800 Mannheim 31, Germany. 

Fig. 1. oppositepage) Nonradioactive in-situ hybridization on wax-embedded tissues (A-C) and frozen tissue (D) in combination with immunohistochemical staining. Blue staining represents in-situ hybridization signal andred/greenis the reaction following immunohistochemical... 

Rex M, Scotting PJ (1994) Simultaneous detection of RNA and protein in tissue sections by nonradioactive in situ hybridization followed by iinmunohistochemistry. Biochemica [Boehringer Mannheim] 3 24-26. 

Xu, X-C, Clifford, J. L., Hong, W. K., and Lotan, R (1994) Detection of nuclear retinoic acid receptor mRNA in histological tissue sections using nonradioactive in situ hybridization histochemistry. Diag. Mol. Pathol. 3, 122-131. 

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