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Nitrogen-15 analysis, bones

We proposed to study diet and health by combining bone chemistry and histomorphometry. Diet would be determined by analysis of stable isotopes of carbon and nitrogen in bone protein and some preserved hair. In addition, trace elements would be quantitatively analyzed in preserved bone mineral. Abonyi (1993) participated in the study by reconstructing the diet from historical sources and analyzing various foods. Having analyzed human tissues for stable isotopes and trace elements, and foods for the same variables, we hoped to learn more about 19th century diet in southern Ontario, and at the same time, learn more about paleodiet reconstruction. [Pg.3]

Stable isotope analyses of the organic fraction of bone and of food samples was carried out on a Micromass Prism Mass Spectrometer in the Stable Isotope Laboratory, Department of Physics, University of Calgary, under the direction of H.R. Krouse. Collagen samples were combusted in a Carlo Erba gas analyser which provides information on the carbon and nitrogen content of the samples andintroduces Nior CO gases into the mass spectrometer for analysis of nitrogen or carbon stable isotopes, respectively. [Pg.4]

Styring, A. K., Sealy, J. C. and Evershed, R. P. (in press) Resolving the bulk 81SN values of ancient human and animal bone collagen via compound specific nitrogen isotope analysis of constituent amino acids. Geochimica et Cosmochimica Acta. [Pg.432]

In conclusion, nitrogen/carbon ratios combined with quantitative amino acid analyses could determine the level of impurities that may co-exist with fossil bone collagen and could help in selecting the optimum method of collagen separation. An extraction method may be successful in some cases but could fail to remove the impurities from bone collagen in other samples. Chemical analysis of the impurities and their radiocarbon dates also should be obtained. [Pg.116]

Minor element contents and isotopic ratios are used for physiological purposes. Sr/Ca ratios in bone and teeth are said to reflect the diet of the animal (herbivores versus carnivores). Stable isotope analysis of teeth and bones provides direct information on the lifetime diets the nitrogen isotopes reflect the trophic level of the protein that has been consumed. Within an ecosystem, they can identify herbivores and carnivores, while the carbon isotopes tell mainly about the amount of protein in the diets from terrestrial versus marine ecosystems. [Pg.331]

A hydrocarbon fuel is burned with bone-dry air in a furnace. The stack gas is at 116 kPa and has a dew point of 47°C. The Orsat analysis of the gas shows 10 mol % carbon dioxide the balance consists of oxygen and nitrogen. What is the ratio of hydrogen to carbon in the hydrocarbon fuel ... [Pg.354]

You will need mononuclear cells from peripheral blood or bone marrow collected in heparinized glass tubes and isolated on Ficoll-Paque as rapidly as possible. After collecting the interphase cells, they are washed and resuspended in the buffered medium supplemented with 10% FCS (= assay medium) and kept on melting ice until analysis. Alternatively, the cells are frozen in medium with 10% DSMO and at least 10% FCS in liquid nitrogen according to protocols for cryopreservation (see Note 1). [Pg.55]

Fig. 1.9 Tamsin O Connell and students preparing samples of bone and hair for carbon and nitrogen isotopes analysis in the Dorothy Garrod Laboratory for Isotopic Analysis at Cambridge University... Fig. 1.9 Tamsin O Connell and students preparing samples of bone and hair for carbon and nitrogen isotopes analysis in the Dorothy Garrod Laboratory for Isotopic Analysis at Cambridge University...
The ratio of carbon to nitrogen in a sample is used as a measure of the quality of preservation in bone and a means to determine if samples are reliable for the analysis of collagen for radiocarbon dating and dietary analysis using light isotopes. The ratio of C N in living bone is 3.2 a ratio between 2.8 and 3.5 has been suggested as indicative of fossil bone sufficiently well preserved for analysis. [Pg.89]

Ambrose, S.H. 2000. Controlled diet and climate experiments on nitrogen isotope ratios of rat bone collagen, hair and muscle. In Biogeochemical Approaches to Paleodietary Analysis, S.H. Ambrose, and M.A. Katzenberg (eds.), pp. 243-259. New York Kluwer Academic/Plenum. [Pg.275]

Lynch. B.M.. and R.W. Jeffries. 1982. A comparative analysis of the nitrogen content of bone as a means of establishing a relative temporal ordination of prehistoric burials. Journal of Archaeological Science 9 381-390. [Pg.289]


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