4-/>-Nitrobenzyl pyridine

Nitrobenzyl)pyridine (PNBP) [1083-48-3] M 214.2, m 70-71 , pKgj, 5.0. Crystd from cyclohexane. 

The fact that the binder used in the layer can affect the reagent is shown in the monograph on 4-(4-Nitrobenzyl)pyridine reagent. It is not possible to employ this reagent on Nano-SIL C 18-100 UV254 plates (Macherey-Nagel) because the whole surface of the layer is colored bluish-violet. The corresponding water-wettable layers produced by the same manufacturer do not present any difficulties. 

Spray solution I Dissolve 2 —5 g 4-(4-nitrobenzyl)pyridine in 100 ml acetone [7-10],... 

Alcohols 4-(4-Nitrobenzyl)- pyridine Amino compounds, esters and ethers do not interfere, but phenols and acids as well as epoxides, olefins and substances containing labile halogen probably do. [7]... 

To further investigate the role of the liver in brevetoxin metabolism, PbTx-3 was studied in the isolated perfused rat liver model (27, 28). Radiolabeled PbTx-3 was added to the reservoir of a recirculating system and allowed to mix thoroughly with the perfusate. Steady-state conditions were reached within 20 min. At steady-state, 55-65% of the delivered PbTx-3 was metabolized and/or extracted by the liver 26% remained in the effluent perfusate. Under a constant liver perfusion rate of 4 ml/min, the measured clearance rate was 0.11 ml/min/g liver. The calculated extraction ratio of 0.55 was in excellent agreement with the in vivo data. Radioactivity in the bile accounted for 7% of the total radiolabel perfused through the liver. PbTx-3 was metabolized and eliminated into bile as parent toxin plus four more-polar metabolites (Figure 3). Preliminary results of samples stained with 4-(p-nitrobenzyl)-pyridine (29) indicated the most polar metabolite was an epoxide. 

By far the most commonly used reagent for detecting and measuring alkylating agents is 4-(4 -nitrobenzyl)pyridine (NBP) [49-53]. This reacts in the following way ... 

NBP, 4-(4-nitrobenzyl)pyridine TFTP, 2,3,5,6-tetrafluorothiophenol DDTC, Diethyldithiocarbamate BSTFA, bis (trimethylsilyl) trifluoroacetamide TFAA, trifluo-roacetic anhydride o-PA, o-phthalaldehyde HFBA, heptafluorobutyric anhydride. 

J. H. Kim and J. J. Thomas, Use of 4-(nitrobenzyl)pyridine (4-NBP) to test mutagenic potential of slow-reacting epoxides, their corresponding olefins, and other alkylating agents. Bulletin of Environmental Contamination and Toxicology, 1992, 49(6), 879-885. 

W. Preibsch, K. D. Hofmann and W. Krafft, Method for determination of alkylating activity in aqueous solutions by reaction with NBP [4-(p-nitrobenzyl)pyridine]. Archiv fuer Geschwulstforschung, 1972, 40(3), 259-262. 

R. Sohr, G. Schmeck, R. Preiss and M. Matthias, Methodology of the colorimetric determination of alkylating cytostatics with 4-(4 -nitrobenzyl)pyridine (NBP) in plasma. Pharmazie, 1982, 37(11), 777-779. 

R. A. Christian, S. K. Chaffee, C. J. Hovick and W. J. Steele, A stable colorimetric assay for cyclophosphamide and its alkylating metabolites based on the alkylation of 4-(4 -nitrobenzyl)-pyridine. Life sciences, 1980, 27(25-26), 2595-2599. 

J. A. Guth and L. P. Manner, 4-(p-Nitrobenzyl)pyridine as a reagent for thin-layer chromatographic determination of various aza heterocycles. Journal of Chromatography, 1967,30(2), 514-521. 

Barbin, A., Planche, G, Croisy, A., Malaveille, C. Bartsch, H. (1977) Detection of electrophilic metabolites of halogenated olefins with 4-(4-nitrobenzyl)pyridine (NBP) or with Salmonella typhimurium (Abstract), In 2nd International Conference on Environmental Mutagens, Edinburgh, July 1977,p. 59... 

Hemminki, K. Falck, K. (1979) Correlation of mutagenicity and 4-(p-nitrobenzyl)-pyridine... 

Aziridines can be detected by a color reaction with 4-(4 -nitrobenzyl)pyridine that was originally developed for the detection and/or determination of a wide range of alkylating substances. 58 It can be adapted to TLC. 59 60 Upon acid hydrolysis of aziridine-2-carboxylic acid containing peptides, as required for amino acid analysis, this amino acid is recovered as serine in varying yields. 47 Configurational characterization of aziridine-2-carboxylates is performed by NMR spectroscopy 47 51 61-63 and X-ray analysis. 61 In addition, optical rotation of the related benzyl Wtritylaziridine-2-carboxylate is also used 64 with [a]D —95.5 (c 1, THF) for the 25-enantiomer 44 as reference. 

Aziridine traces in aqueous solution can be determined by reaction with 4-(/>-nitrobenzyl)pyridine [1085-48-5] and potassium carbonate [584-08-7]. Quantitative determination is carried out by photometric measurement of the absorption of the blue dye formed (367,368). Alkylating reagents interfere in the determination. Aziridine traces in the air can be detected discontinuously by absorption in Folin s reagent (l,2-naphthoquinone-4-sulfonate) [2066-95-5] (369,370) with subsequent chloroform extraction and hplc analysis of the red dye formed (371,372). The detection limit is ca 0.1 ppm. Nitrogen-specific thermal ionization detectors can be used for continuous monitoring of the ambient air. 

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