Nitrobenzyl pyridine Reagent

Reagent for Epoxides e.g. trichothecene-mycotoxins [1 — 6] valepotriates [7,17] Olefins, acetylene derivatives [8] 4-Hydroxycumarin, anthraquinone [8] Alkylating agents [9-12] NOz 

Dipping solution I Dissolve 9 g 4-(4-nitrobenzyl)pyridine (NBP) and 90 mg bu-tylhydroxytoluene (BHT) in 90 ml chloroform. 

Dipping solution II Dissolve 24 ml tetraethylene pentamine and 60 mg butyl-hydroxytoluene in 36 ml dichloromethane. 

Spray solution I Dissolve 2 —5 g 4-(4-nitrobenzyl)pyridine in 100 ml acetone [7-10], 

Spray solution II Dissolve 92 mg potassium hydrogen phthalate in 100 ml water and adjust the pH to 5.0 with sodium hydroxide solution (1 mol/l). Dissolve 5 g sodium perchlorate in this solution [9]. 

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The fact that the binder used in the layer can affect the reagent is shown in the monograph on 4-(4-Nitrobenzyl)pyridine reagent. It is not possible to employ this reagent on Nano-SIL C 18-100 UV254 plates (Macherey-Nagel) because the whole surface of the layer is colored bluish-violet. The corresponding water-wettable layers produced by the same manufacturer do not present any difficulties. 

By far the most commonly used reagent for detecting and measuring alkylating agents is 4-(4 -nitrobenzyl)pyridine (NBP) [49-53]. This reacts in the following way ... 

J. A. Guth and L. P. Manner, 4-(p-Nitrobenzyl)pyridine as a reagent for thin-layer chromatographic determination of various aza heterocycles. Journal of Chromatography, 1967,30(2), 514-521. 

Aziridine traces in aqueous solution can be determined by reaction with 4-(/>-nitrobenzyl)pyridine [1085-48-5] and potassium carbonate [584-08-7]. Quantitative determination is carried out by photometric measurement of the absorption of the blue dye formed (367,368). Alkylating reagents interfere in the determination. Aziridine traces in the air can be detected discontinuously by absorption in Folin s reagent (l,2-naphthoquinone-4-sulfonate) [2066-95-5] (369,370) with subsequent chloroform extraction and hplc analysis of the red dye formed (371,372). The detection limit is ca 0.1 ppm. Nitrogen-specific thermal ionization detectors can be used for continuous monitoring of the ambient air. 

The use of 4-(p-nitrobenzyl)pyridine (NBP) as an analytical reagent for the determination of alkylating agents [59,60] is based on the series of reactions presented in scheme IIA. Melphalan is treated at 80—100° with a large excess of the chromogenic reagent to... 

For sensitive and relatively specific detection of phosgene, a spectrophotometric GC-MS technique has been used that utilizes a reagent consisting of 0.4% of nitrobenzyl pyridine and 0.5% of sodium acetate in ethanol (Dangwal, 1994). The method is so sensitive as to detect phosgene at a concentration of 0.1 microgram/ml in the sampling solution with the coefficient of variation (CV) of 4.5%. 

The hydrophobic portion of the reagent, but not adenine, appears to be essential for the alkylation of the adenosine deaminase active site since benzylbromoacetate irreversibly inactivates the enzyme whereas the site is inaccessible to haloacetate and its amide. Benzylbromoacetate and 9-(p-bromoacetamidobenzyl) adenine are only 4 and 2.5 more reactive, respectively, than bromoacetate toward 4-(p-nitrobenzyl)pyridine. The oxidation of ribose of purine riboside with periodic acid in order to produce carbonyl groups that may react with lysine residue destroys the inhibitory effectiveness of the nucleoside. ... 

The sulfar-containing OP insecticides can be detected eithor with iodine-azide (149) or with potassium iodate (150) reagents. 4-(4 -nitrobenzyl)pyridine-tetraethy)enepentamine reagent is also applied for their detection (21). 

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