Nitric oxide spin probes

LeBrun, N. F., Cheeseman, M. A., Thomson, A. J., Moore, G. L., Anders, S. C., Guest, J. R., and Harrison, P. M. (1993). An epr investigation of non-haem iron sites in Escherichia coli bacterioferritin and their interaction with phosphate—A study using nitric oxide as a spin probe. FEES Lett. 323, 261-266. 

Closely related are the 1-benzylamino-l-deoxylactitol dithiocarbamate salts developed by Eybl and co-workers316 317 for the same purpose. However, the most important application of 175 is, probably, its use as a nontoxic, water-soluble nitric oxide probe in vivo. In view of the central importance that this gaseous free-radical species plays in regulating a broad range of important biological functions, its detection and quantification near its site of production and action is of prime importance. For this purpose, the ferrous salt of MGD, which forms a stable water-soluble mononitrosyl iron-dithiocarbamate complex (176) with a characteristic electron spin resonance (ESR) spectrum at room temperature, is currently used.318-323... 

O Keeffe, D. H., Ebel, R. E., and Peterson, J. A. (1978). Studies of the oxygen binding site of cytochrome P-450. Nitric oxide as a spin-label probe. /. Biol. Chem. 253, 3509-3516. Pous, C., Giroud, J. P., Damais, C., Raichvarg, D., and Chauvelot-Moachon, L. (1990). Effect of recombinant human interleukin-1 and tumor necrosis factor a on liver cytochrome P-450 and serum a-l-acid glycoprotein concentrations in the rat. Drug Metab. Dispos. 18, 467-470. 

---