Nicotiana plumbaginifolia

A (1996) Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana , EMBO J, 15, 331-42. 

Qin, X. Q. and J. A. D. Zeevaart (2002). Overexpression of a 9-cA-epoxycarotenoid dioxygenase gene in Nicotiana plumbaginifolia increases abscisic acid and phaseic acid levels and enhances drought tolerance. Plant Physiol. 128(2) 544-551. 

Maestri, E., Restivo, F. M., Gulli, M., and Tassi, F., 1991, Glutamate-dehydrogenase regulation in callus-cultures of Nicotiana plumbaginifolia - effect of glucose feeding and carbon source starvation on the isoenzymatic pattern, Plant Cell Environ., 14 613-618. 

Sims, I. M., Craik, D. J., Bacic, A. (1997). Structural characterization of galactoglucomannan secreted by suspension-cultured cells of Nicotiana plumbaginifolia. Carbohydr. Res., 303, 79-92. 

Fig. 2. Biochemical classification of the nia mutants, mutated domains and intragenic complementation. A collection of Nicotiana plumbaginifolia nia mutants (defective for NR apoenzyme) was tested for NR protein and activities (Cherel et al., 1990) and checked for in vivo and in vitro intragenic complementation (Pelsy Gonneau, 1991). NR protein amounts were measured by a sandwich ELISA test using as first antibody the anti-corn NR monoclonal antibody 96.9.25 (Ch6rel eta/., 1985) shown to be directed against the haem domain (M. Kavanagh, personal communication Meyer etal., 1991). The result is shown here as + or when a positive or negative ELISA test, respectively, was observed. Many class 4 mutants, most probably frameshift and deletion mutants, complement class 3 but not class 2 mutants.

Ch6rel, I., Gonneau, M., Meyer, C., Pelsy, F. Caboche, M. (1990). Biochemical and immunological characterization of nitrate reductase-deficient nia mutants of Nicotiana plumbaginifolia. Plant Physiology 92, 659-65. 

Dorbe, M.-F., Caboche, M. Daniel-Vedele, F. (1992). The tomato NIA gene complements a Nicotiana plumbaginifolia nitrate reductase-deficient mutant and is properly regulated. Plant Molecular Biology 18,.363-75. 

P. (1987). Bromphenol blue nitrate reductase activity in Nicotiana plumbaginifolia. An immunological and genetic approach. Biochimie 69, 735-42. 

Meyer, C., Levin, J.M., Roussel, J.M. Rouze, P. (1991). Mutational and structural analysis of the nitrate reductase haem domain of Nicotiana plumbaginifolia. Journal of Biological Chemistry 266, 20561-6. 

Pelsy, F. Gonneau, M. (1991). Genetic and biochemical analysis of intragenic complementation events among nitrate reductase apoenzyme-deficient mutants of Nicotiana plumbaginifolia. Genetics 127, 199-204. 

Pouteau, S., Cherel, I, Vaucheret, H. Caboche, M. (1989). Nitrate reductase mRNA regulation in Nicotiana plumbaginifolia nitrate... 

Castresana, C., Garcia-Luque, I., Alonso, E., Malik, V.S. Cash-more, A.R. (1988). Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia. The EMBO Journal 7, 1929-36. 

Nardi et al. (1994), by using two inhibitors of auxin (TIBA = 2,3,5-triiodobenzoic acid and PCIB = 4-chlorophenoxy-isobutyric acid), demonstrated in Nicotiana plumbaginifolia that the LMS component of humic matter is the fraction endowed with auxin-like activity, although the pathways followed by IAA and the LMS fraction in inducing their effects may be somewhat different. Humic matter, IAA, and IAA inhibitors stimulated peroxidase activity in tobacco. When the Nicotiana tissues were treated with the humic fraction and IAA, there was a minor polymorphism in the esterase isoenzymes. The presence of both TIBA or PCIB together with LMS or IAA restored the esterase profile obtained from control tissues. 

The BR effect on growth was shown with other species. In Nicotiana plumbaginifolia L. we have obtained similar results for cell enlargement in liquid cell suspensions, no effect on multiplication, but stimulation of plating efficiency at the BR concentration of 1 /xM. 

Tobacco (Nicotiana plumbaginifolia) desaturase activity, compensation effects [ RuCl2(TPPMS)2 3] [214]... 

A new solasodine glycoside, solaplumbin, has been isolated from aerial parts of Nicotiana plumbaginifolia. Structure (25) was proposed for the glycoside on the basis of microanalytical data and the degradations outlined below. 

If physiological approaches are to be used to resolve issues about the relationship between calcium and cytokinins, then a system is needed in which one can add hormone and rapidly measure local Ca" " transients or waves. Experimental conditions need to be found to avoid mechanical disturbances known to induce strong calcium responses as measured by aequorine luminescence [70]. So far, our attempts to measure Ca transients using aequorine luminescence in Nicotiana plumbaginifolia cells and seedlings have been unsuccessful (Faure and Howell, unpublished data). 

Dismutase, superoxide (Nicotiana plumbaginifolia clone pSOD3 copper-zinc protein moiety reduced) 1623b, 4249... 

Perez, C., B. Michelet, V. Ferrant, P. Bogaerts, and M. Boutry Differential expression within a three-gene subfamily encoding a plasma membrane hydrogen ion-AT-Pase in Nicotiana plumbaginifolia, J. Biol. Chem. 267 (1992) 1204-1211. 

Santoso, D. and R. Thornburg Uridine 5-monophos-phate synthase is transcriptionally regulated by pyrimidine levels in Nicotiana plumbaginifolia Plant Physiol. 116 (1998)815-821. 

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