Negative regulatory element

Izumi, R., Yamada, T., Yoshikai, S., Sasaki, H., Hattori, M., Sakaki, Y. (1992). Positive and negative regulatory elements for the expression of the Alzheimer s disease amyloid precursor-encoding gene in mouse. Gene 112, 189-195. 

Figure 4. Transcriptional regulatory sequences of the mouse c-mos gene. Transcription in oocytes and spermatocytes initiates 53 and approximately 280 base pairs upstream of the c-mos ATG, respectively. Efficient transcription in oocytes requires an initiator (Inr)-I ike sequence located downstream of the transcription start site. A negative regulatory element (NRE) located upstream of the spermatocyte promoter acts to suppress c-mos transcription in somatic cells.

Figure 6. The c-mos negative regulatory element (NRE). Nucleotide positions of the NRE are shown relative to the spermatocyte transcription start site, taken as 280 base pairs upstream of the c-mos ATG (see Fig. 4). The endpoints of the NRE are defined by deletions that allow c-mos expression in NIH 3T3 and other somatic cells. Mutations of the sequences designated by boxes 1,2, and 3 also allow c-mos transcription in NIH 3T3 cells, indicating that these sequences represent functional elements within the NRE. Boxes 1 and 2 are similar to sequences upstream of the protamine (Prot) promoter that inhibit in vitro transcription in HeLa cell extracts. A sequence just upstream of box 2 is also similar to a putative repressor-binding site in the regulatory region of Pgk2.

GanB, R., Schiitz, G., and Beermann, F. (1994a). The mouse tyrosinase gene promoter modulation by positive and negative regulatory elements. J. Biol. Chem. (in press). 

Castresana, C., Garcia-Luque, I., Alonso, E., Malik, V.S. Cash-more, A.R. (1988). Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia. The EMBO Journal 7, 1929-36. 

Chang MS, Yoo HY, Rho HM. Positive and negative regulatory elements in the upstream region of the rat CuTZu-superoxide dismutase gene. Biochem J 1999 339(pt 2) 335-341. 

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