Gene complementation

Islas, S., Becerra, A., Luisi, P. L., and Lazcano, A. (2004). Comparative genomics and the gene complement of a minimal cell. Orig. Life Evol. Biosph., 34 (1-2), 243-56. 

Kindt, T.J., Gris, C., Guenet, J.L., Bonhomme, F., Cazenave, P.-A. (1985). Lambda light chain constant and variable gene complements in wild-derived inbred mouse strains. Eur. J. Immunol. 15,... 

Dorbe, M.-F., Caboche, M. Daniel-Vedele, F. (1992). The tomato NIA gene complements a Nicotiana plumbaginifolia nitrate reductase-deficient mutant and is properly regulated. Plant Molecular Biology 18,.363-75. 

CM Fraser, JD Gocayne, O White, MD Adams, RA Clayton, RD Fleishmann, CJ Bult, AR Kerlavage, G Sutton, JM Kelley, JL Fritchman, JF Weidman, KV Small, M Sandusky, J Fuhrmann, D Nguyen, TR Utterback, DM Saudek, CA Phillips, JM Merrick, J-F Tomb, BA Dougherty, KF Bott, P-C Hu, TS Lucier, SN Peterson, HO Smith, CA Hutchison, JC Venter. The minimal gene complement of Mycoplasma genitalium. Science 270 397-403, 1995. 

Merrick, et al. The minimal gene complement of Mycoplasma genitalium. Science 270 397-403, 1995. 

The complete gene complement of any organism, contained in a set of chromosomes in eukaryotes, a single chromosome in bacteria, or a DNA or RNA molecule in viruses the complete set of genes inside the cell or... 

In the second approach, herbicide-resistance mutations in the Arabidopsis ALS gene were studied in E. coli. To do this, wild type and mutant Arabidopsis genes were functionally expressed in E. coli, such that the plant genes complemented a branched chain amino acid auxotrophy in the bacteria (Smith et al. 1989, PNAS in press). ALS enzyme assays on extracts prepared from E. coli expressing the mutant Arabidopsis gene indicated that the mutant enzyme is resistant to sulfonylurea herbicides but is sensitive to the imidazolinone herbicide imazaquin. This selective... 

Complementation analysis determines whether recessive mutations are in the same or different genes. Complementation tests in yeast are performed by mating haploid a and a cells carrying different recessive mutations to produce diploid cells. In the analysis of cdc mutations, pairs of different haploid temperature-sensitive cdc strains were systematically mated and the resulting diploids tested for growth at the permissive and nonpermissive temperatures. In this hypothetical example, the cdcX and cdcY mutants complement each other and thus have mutations in different genes, whereas the cdcX and cdcZ mutants have mutations in the same gene. 

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