Neomycin resistance

Within the first days under selecting conditions you should observe cell death or an appearance change in a part of the colonies. This serves as an indicator that G418 treatment is deleting the cells which are not expressing the neomycin resistance gene. 

Retrovirus vectors were subjected to the first clinical trial on human gene therapy to correct adenosine deaminase (ADA) deficiency (32). White-blood cells isolated from patients were infected ex vivo with an MLV-based vector expressing ADA and a neomycin marker gene. After selection with G418, neomycin-resistant cells were isolated and reintroduced into patients. The treatment improved the physical condition of the patients and the ADA-containing provirus was stable in the blood for several years. 

Gene transfer of mouse and human CD34+ HSCs, which were genetically modified by a retroviral virus encoding ADA cDNA and the neomycin-resistance marker gene, have been extensively characterized in mouse models without reports of toxicity. Furthermore, none of the preclinical studies for ADA-SCID have demonstrated evidence of cancer in animals treated with this same gene transfer approach [515137], [666652], [666655]. 

Flermonat, P. L. and Muzyczka, N. (1984). Use of adeno-associated virus as a mammalian DNA cloning vector Transduction of neomycin resistance into mammalian tissue culture cells. Proc. Natl. Acad. Sci. USA 81, 6466-6470. 

Targeting vector A vector containing a positive selection marker (e.g., neomycin-resistance gene), negative selection marker (thymidine kinase gene) and a multiple cloning site. 

Nrg1tmlcbm Targeted mutation 1, Carmen Birchmeier A neomycin resistance gene replaces exons 9,10, and 11, which encode the carboxy-terminus of the EGF domain of all known Nrgl variants... 

Interaction between -lactamase and intrinsic resistance Resistance to aminoglycosides Resistance to kanamycins Resistance to neomycins Resistance to streptomycin Resistance to gentamicin Resistance to lividomycin Resistance to tobramycin Resistance to BB-K8 Resistance to chloramphenicoi... 

R factor mediated kanamycin resistance in strains of Ps. aeruginosa has been observed by a number of workers. The pseudomonas R factor RPl (142) carries the neomycin-kanamycin phosphorylating enzyme [205]. A study of the prevalence of R factor carrying strains of Ps. aeruginosa in Japanese hospitals showed a number of strains capable of transferring both kanamycin and neomycin resistance [206]. 

Dulon D, Ryan AF. The bacterial Neo gene confers neomycin resistance to mammalian cochlear hair cells. Neuroreport 1999 10(6) 1189-93. 

Tourdot-Marechal, R., Fortier, L.-P., Guzzo, J., Lee, B., and Divies 1999. Acid sensitivity of neomycin-resistant mutants of Oenococcus oeni A relationship between reduction of ATPase activity and lack of malolactic activity. FEMS Microbiol. Lett. 178, 319-326. 

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