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Electrospray, nanoflow

Nanoflow electrospray A form of electrospray ionization, carried out at flow rates of the order of nlmin ... [Pg.308]

Mo, W., Sakamoto, H., Nishikawa, A., Kagi, N., Langridge, J.I., Shimonishi, Y., and Takao, T., Structural characterization of chemically derivatized oligosaccharides by nanoflow electrospray ionozation mass spectrometry, Anal. Chem., 71, 4100, 1999. [Pg.68]

Janini, G.M., Comads, T.P., Wilkens, K.L., Issaq, H.J., Veenstra, T.D. (2003). A sheathless nanoflow electrospray interface for on-line capillary electrophoresis mass spectrometry. Anal. Chem. 75, 1615-1619. [Pg.382]

Nanoelectrospray ionization (nanoESI), also known as nanospray, nanoflow electrospray, and micro-electrospray, is a low flow/high sensitivity approach to ESI. NanoESI15 is a slight variation on ESI such that the spray needle has been made very small and is positioned close to the entrance of the vacuum of the mass spectrometer and the mass analyzer (Figure 6). This greatly reduces required sample amounts allowing nanoliter flow rates and femto-mole sample consumption. The end result is increased efficiency since the flow rates for... [Pg.682]

The liquid volume of a sample required for analysis depends on the ionization technique, MALDI or ESI, and the introduction technique (see Table 4.1). The following statements assume that we are analyzing a sample near the detection limit of the analyte in a specific mass spectrometer. For MALDI-MS, the researcher typically spots 0.1 to 1 jL onto the MALDI sample plate. Thus, a minimum starting volume of 1 of 5 jL of sample is recommended. For ESI, the required sample volume is primarily dependent on the sample introduction technique. If the researcher uses a nanoflow electrospray technique, capillary EC, or capillary electrophoresis, then typically a l-pL voliune is required. However, larger sample volumes are recommended for ease of handhng. If the voliune is small, then the analysis may be limited to one experiment when additional MS or MS-MS experiments are desired. For higher flow rate ESI sources, the researcher should supply 50 pL or more for direct infusion experiments or for loading 5 to 20 pL onto an analytical EC column. [Pg.81]

Triversa Nanomate or any other nanoflow electrospray ionization source... [Pg.169]

Chen Z, Boggess B, Chang HC (2007) Open-tubular capillary electrochromatography-mass spectrometry with sheathless nanoflow electrospray ionization for the analysis of amino acids and peptides. J Mass Spectrom 42(2) 244-253... [Pg.268]

Electrospray appears to be useful especially when dealing with compounds that give poor APCI spectra due to thermal degradation (such as glucuronide conjugates). Meanwhile, electrospray and its derivative, nanoflow electrospray , have developed a niche in the analysis of proteins. They are especially useful as an ionization method combined with capillary electrophoresis, and for offline analysis of spots retrieved from chromatographic plates. [Pg.2911]

Ionization technique FAB MALDI-ToF Electrospray Nanoflow electrospray... [Pg.2915]

Janini GM, Conrads TP, Wilkens KL, Issaq HJ, Veenstra TD (2003) A sheathless nanoflow electrospray interface for on-... [Pg.184]

Figeys, D., and Aebersold, R. (1998). Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry. Anal. Chem. 70, 3721-3727. [Pg.520]

Very low flow electrospray is called nanoelectrospray [26] where the samples are infused into the mass spectrometer at the nanoliter flow rate range. The infusion of a few microliters will result in a stable signal for more then 30 min, using pulled capillaries or chip-based emitters [27]. With infusion, signal averaging allows to improve the limit of detection in tandem mass spectrometry. Nanoelectrospray is particularly important in combination with nanoflow liquid chromatography or chip-based infusion for the analysis of peptides and proteins. [Pg.14]

Nanoflow HPLC—HPLC system with accurately controlled reciprocating and syringe pumps designed to use capillary and small diameter, high-resolution columns as front ends for electrospray and nanospray mass spectrometer interfaces. [Pg.217]

Commercially, Agilent Technologies produces chips for both direct infusion into a mass spectrometer and for HPLC-MS applications. The chips accommodate nanoflow rates with an electrospray ionisation source, are about the size of a credit card and are rensable. The infusion chip is for collecting direct MS or tandem MS data. The protein HPLC chip has both a sample enrichment and CIS separation column on the chip, as well as the connections and spray nozzles for electrospray. There are also a small molecule chip and a glycan chip. The chip being used is placed in a Chip Cube MS interface which positions the sprayer tip perpendicular to the MS inlet (Figure 10.7). [Pg.267]

Capillary IC features a flow rate in the range of 10-30 pF/min, thus requiring modifications and reoptimization of existing electrospray interfaces that are usually optimized for either analytical flow (100 pF/min to several milliliters/minute) or nanoflow (<1 pF/min) rates. The optimization of interface parameters such as probe temperature, nebulizer gas, needle voltage, type of desolvation solvent, and the flow rate plays a critical role in establishing instrument sensitivity. When capillary IC is operated at a flow rate of 10-20 pF/min, a probe temperature of 300 °C, a needle voltage of 3 kV, a nebulizer gas pressure of 65 psi, and the use of... [Pg.901]

One of the major attractions of ESI is its ability to serve as an interface between liquid chromatography and mass spectrometry. There are currently a number of low-flow HPLC systems on the market that are compatible with electrospray, microspray, and nanospray sources. Capillary HPLC systems are interfaced with electrospray conducted in the pL/min flow regime, while nanoflow systems can accommodate nL/min flow rates. When electrospray is coupled with conventional HPLC, it is necessary to accommodate a higher sample flow rate ( 0.1—2mL/min) than normal electrospray can tolerate. To facilitate operation at these higher flow rates, a technique called pneumatically assisted electrospray or ion spray is employed, in which sample nebulization by aflow of gas is used to stimulate a more... [Pg.67]


See other pages where Electrospray, nanoflow is mentioned: [Pg.83]    [Pg.73]    [Pg.74]    [Pg.202]    [Pg.2911]    [Pg.2915]    [Pg.83]    [Pg.73]    [Pg.74]    [Pg.202]    [Pg.2911]    [Pg.2915]    [Pg.162]    [Pg.104]    [Pg.95]    [Pg.71]    [Pg.95]    [Pg.71]    [Pg.95]    [Pg.955]    [Pg.1566]    [Pg.267]    [Pg.944]    [Pg.63]    [Pg.55]   
See also in sourсe #XX -- [ Pg.104 , Pg.252 ]

See also in sourсe #XX -- [ Pg.104 , Pg.252 ]




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