Na-deoxycholate

Worms washed to remove free label. Homogenised in buffer + enzyme inhibitors + Na deoxycholate. Extracted at 4°C and centrifuged. Radioactivity counted... 

Polyoxyethylene-9-lauryl ether (laureth-9), Brij 35, Brij 96, polysorbate 80, quillaja saponin, dodecylmaltoside Na glycocholate, Na taurocholate, Na deoxycholate,... 

Fig. 1. Time course of ADP-ribosylation of Rho. 1 ng of recombinant GST-Rho (fusion protein) was incubated with 20 (iM of [ P]-NAD and 3ng of the wild type ( ) or the E173Q (o) mutant of C3 exoenzyme in a total volume of 50 nl of ADP-ribosylation buffer, as described in the text, at 30°C for times indicated. The reaction was terminated by the addition of trichloroacetic acid and Na deoxycholate. After centrifugation, the pellets were subjected to SDS polyacrylamide gel electrophoresis, followed by staining with Coomassie Brilliant Blue and autoradiography. P-ADP-ribosylation of Rho was quantified by cutting out the radiolabeled GST-Rho bands and determining their radioactivities. The relative ADP-ribosylation was expressed as the ratio of the P incorporation into GST-Rho at each point to the maximal P incorporation by the wild type C3 exoenzyme... 

Fig. 3. Autoradiogram of P-ADP-ribosylation of Rho in Swiss 3T3 cell homogenate. Swiss 3T3 cells were treated with buffer alone (lane 1), 30 ng/ml of the wild type (lane 2) or the E173Q mutant (lane 3) of C3 exoenzyme for 72 h in DMEM containing 10 % fetal bovine serum. The cells were washed twice in phosphate-buffered saline (PBS), and incubated with 0.05 % (w/v) trypsin in PBS. The detached cells were collected and suspended in ADP-ribosylation buffer containing [ P]-NAD, followed by sonication. The homogenates were incubated with 100 ng of the wild type C3 enzyme at 30°C for 2 h. The reaction was terminated by the addition of trichloroacetic acid and Na deoxycholate. The pellets were subjected to 12 % SDS polyacrylamide gel electrophoresis and autoradiography. The positions of molecular weight markers are indicated on the left. The position of ADP-ribosylated Rho is indicated by an arrow...

Cells are lysated by an aqueous solution of 0.1% Na-deoxycholate (Sigma-Aldrich BV, Cat. D-6750). 

The effect of various absorption enhancers on insulin transport across the rectal membrane of albino rabbits was examined by an in vitro Us sing chamber method [18]. Insulin was unable to cross the rectal mucosa without absorption enhancers, but its transport was improved in their presence. Among these enhancers, Na glyco-cholate (NaGC) was more effective than Na taurocholate (NaTC), but less effective than Na deoxycholate (NaDC) and poly-... 

Na-deoxycholate 415 700- 9,000 0.117(1-2.7) 0.778 Forms giant micelles when pH < 7.8. Precipitates when pH <6.9. CMC decreases with increasing salt concentration. Precipitates with Ca, Mg ". 

Wash the beads four times with PBS containing 1% NP-40 (instead of PBS, RIPA buffer may be used for more stringent washing). Wash the beads two times with a buffer containing 10 mM Tris-HQ, pH 8.0, 0.25 M LiQ, 0.5% NP-40, 0.5% Na deoxycholate, 1 mM EDTA. Wash the beads two times with TE. Resuspend the beads in 120 fil of TE. Remove 10 /il for Western analysis. Remove 10 fil and count [ Hjthymidine. This will give an idea of how efficient the immunoprecipitation was, and should show a large difference between preimmune and anti-SATBl serum. 

From Na-deoxycholate solubilized brain microsomes the fraction that precipitates between 45 and 60 per cent ammonium sulfate saturation was obtained. This fraction contains ATPases that can be activated with Mg2+ or Ca2+ or Mn2+ in the presence of EDTA and ouabaine (Table 4). In the presence of EGTA (ethanedioxy bis (ethyl amine tetra-acetic acid)) but not ouabaine, and Mg2+ the addition... 

R. Kanamoto, N. Azuma, H. Suda, T. Seki, Y. Tsuchihashi and K. Iwami, Elimination of Na" -dependent bile-acid transporter from small intestine by ileum resection increase colonic tumourigenesis in the rat fed deoxycholic acid, Cancer Lett., 1999, 145, 115. 

SYNS DEOXYCHOLATE SODIUM DEOXYCHOLIC ACID SODIUM SALT DIHYDROXY 3-12 CHOLANATE de Na (FRENCH) (3-a,5-3,12-a)-3,12-DIHYDROXY-CHOLAN-24-OIC ACID MONOSODIUM SALT 3-a,12-a-DIHYDROXY-5-3-CHOLAN-24-OIC ACID SODIUM SALT NA-DESOXYCHOLAT (GERMAN) NATRIUM-3-a,12-O-DIHYDROXYCHOLANAT (GERMAN) SODIUM DEOXYCHOLATE SODIUM DEOXYCHOLIC ACID... 

From refs. 50 and 52 extrapolated to infinite dilution. Data for lithocholate based on 1.6 cm /mole difference between cholate and deoxycholate [52], Anionic data based on additivity. <(>J (K ) = 3.6 cmVmole <)>° (Na ) = - 6.6 cm mole. 

The liver secretes a elear, golden yellow viscous liquid known as bile . It is stored in gall bladder and is solely useful for the digestive system. It mainly consists of the inorganie ions like HCO3, Cl Na , K, etc., in addition to organic compoimds such as bile aeids, bile pigments, liquid fatty acids and cholesterol. Cholic Acid Deoxycholic Acid Chenodeoxycholic Acid. 

Porcine femur (trabecular bone) N aN3+chloroform/methanol+ Triton X-100+sodium deoxycholate Human ASCs NA Kedong et al. (2014)... 

The spectrum of deoxycholic acid was studied in CD3OD and Na deoxy-cholate in DoO. Peaks due to the protons attached to the steroid nucleus at positions C 2 (about 6.0 ppm) and C3 (about 6.4 ppm) are present. 

Fig. 38. The effect of dihydroxy and trihydroxy bile salts on the solubility of lithocholate. The upper and lower sets of three curves (Na salts, 120 mM Na salts, 25 mA/) represent the solubility of NaL as a function of temperature in varying amounts of sodium chenodeoxycholate (J), sodium deoxy-cholate (O). and sodium cholate ( ). Total bile salt concentrations are 120 mM in upper three curves and 25 mAf in lower three curves. The middle set of three curves (K salts, 120 mM) represent solubility of KL in potassium chenodeoxycholate (J), potassium deoxycholate (O), and potassium cholate ( ), at a total bile salt concentration of 120 mM (45).

Figure 7.42 Solubilization of microsomal constituents by increasing concentrations of (a) deoxycholate (b) cetyltrimethylammonium bromide Protein (O), chol terol (A), phospholipid phosphorus ( ), and Na -ion-stimulated adenosine triphosphatase ( ). Diagrams show the percentage of this constituent in the supernatant of the microsomal suspensions after treatment, as described by Bradford et al [194].

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