Mucoid

Although exopolysaccharides do not normally have a structural role, they do form structures that can be detected by either light or electron microscopy. Exopolysaccharides may form part of a capsule closely attached to the microbial cell surface, or appear as loose slime secreted by the cell but not directly attached to it mucoid Exopolysaccharide producing cells usually form mucoid colonies on solid media and colonies liquid cultures of these cells may become very viscous. However, growth conditions can... 

In mart, an overlooked feature is the occurrence of mucoid-like plugs in the foetal nostrils (Schaeffer, 1910). The presence of this blockage can be confirmed by endoscopic inspection in utero these plugs seem likely to affect free amniotic flow, since they appear to be reinforced by a folded membranous gathering at the nasal vestibule (PI. 4B). A degree of restriction of fluid access to the VN aperture, which is immediately caudal to the nostril aperture, and is patent in foetal life, may be a protective feature (Jordan, 1972). The timing of the dissolution of these sealant devices prior to parturition is regrettably not known. 

Takami S., Getchell M. and Getchell T. (1995). Resolution of sensory and mucoid glycoconjugates with terminal O-galactose residues in the mucomicrovillar complex of the vomeronasal sensory epithelium by dual confocal laser-scanning microscopy. Cell Tiss Res 280, 211-216. 

C. G. Gahmberg and L. C. Anderson, Leucocyte surface origin of human alpha-1-acid glycoprotein (oroso-mucoid), J. Exp. Med, 148, 507-521 (1978). 

Alkali on the IV-Acetylhexosamines and the Specific Blood-Group Mucoids. Biochem. J. 51, 379 (1952). 

Phaeococcomyces exophialae forms slimy, mucoid, slow-growing, smooth colonies that are grayish black. Budding yeast cells which are at first subhyaline are abundant. With age, some of the cells become darker, with thickened cell walls. Some pseudohyphae usually are present. Hyphal development may become dominant in some isolates of this species with subsequent subculture. 

Extracellular material of a slimy or gelatinous nature is formed by many bacteria, especially those producing mucoid growths. This material may remain firmly adherent as a discrete covering layer on each cell, or it may part freely from the cells. In the former case it is known as a capsule in the latter, as free slime or gum. 

Malhotra S et al. Proteome analysis of the effect of mucoid conversion on global protein expression in Pseudomonas aeruginosa strain PAOl shows induction of the disulfide bond isomerase, dsbA. J Bacterid 2000 182 6999-7006. 

The supernatant is discarded and the tube with the specimen is resuspended using a vortex. CytoLyt Solution is added to specimen at a ratio of 1 2 (specimensolution). If the specimen is bloody or mucoid, a few more milliliter of CytoLyt are added. The specimen is vortexed again and centrifuged at 2500 rpm for 5 min. 

Using a ThinPrep Processor 2000, select the proper sequence for non-gynecologic specimens (number 2 if mucoid, number 3) to prepare the slide. Please see the next ThinPrep Non-Gynecologic Preparation for details. 

Select the proper sequence for non-gynecologic specimen (number 2 for mucoid specimens number 3). 

Gupta, B. L. (1989). The relationship of mucoid substances and ion and water transport, with new data on intestinal goblet cells and a model for gastric secretion. In Mucus and Related Topics, eds. Chantler, E. and Ratcliffe, N. A., The Company of Biologists, Cambridge, pp. 81-110. 

Hamelin, C., and Y. S. Chung. Characterization of mucoid mutants of Escherichia coli K-12 isolated after exposure to ozone. J. Bacteriol. 122 19-24, 1975. 

---