MS protocols for

Various GC-MS protocols for additive analysis have been developed. Kawamura et al. [247] have presented... 

Lately, electrospray ionisation technique (ESI-MS) which is compatible with RP-HPLC has been routinely used. This allows labile molecules to be studied intact. Sample molecules are simultaneously nebulised and ionised at atmospheric pressure in the presence of several thousand volts. The resulting ions can be multi-protonated (multiply charged) and relatively stable. This mode of ionisation has recently been used in the development of RP-HPLC coupled with positive ion ESI-MS and ion-trap MS protocols for the identification and... 

Baglan N, Cossonnet C, Trompier F, Rite J and Berard P (1999) Implementation of ICP-MS Protocols for Uranium Urinary Measurements in Worker Monitoring. Health Physics 77 50-58. 

Baglan, N., Cossonnet, C.,Trompier, F., Ritt,J., and Berard, P. (1999). Implementation of ICP-MS protocols for uranium urinary measurements in worker monitoring. Health Phys. 77(4), 455. 

Development and validation of protocols for solid-phase extraction coupled to LC and LC-MS have been described [136]. Hennion [137] has reviewed online SPE-HPLC coupling followed by various detection modes. 

The protocol for using isobaric tags differs from that described previously for the ICAT or ECAT type reagents. In the following method, the proteins are denatured and the disulfides reduced and then alkylated to block them permanently. This eliminates disulfide re-association and also prevents the isobaric tags from forming thioester modification with cysteine thiols. Next, the proteins are digested with trypsin and then modified with an isobaric tag. Each sample is labeled with a different isobaric compound so that the samples can be differentiated upon MS/MS analysis. 

The sample materials from which proteins for proteomics studies may be extracted include fresh or snap-frozen cells from varied sources such as biological fluids, (serum, urine, plasma) and solid tissues such as biopsy specimens. Moreover, proteins isolated from ethanol-fixed paraffin-embedded tissues can be utilized for MS analysis.2 Protocols for the identification of proteins from formalin-fixed paraffin-embedded (FFPE) tissues have been recently developed.3 4 FFPE materials are the most common forms of biopsy archives utilized worldwide, and represent an important advancement for the large-scale interrogation of proteins in archival patient-derived materials. Finally, laser capture microdissected tissues have been successfully used for MS analysis.45... 

J. Gobom, M. Schuerenberg, M. Mueller, D. Theiss, H. Lehrach, and E. Nordhoff. a-Cyano-4-hydroxycinnamic Acid Affinity Sample Preparation. A Protocol for MALDI-MS Peptide Analysis in Proteomics. Anal. Chem., 73(2001) 434-438. 

E. Nordhoff, M. Schurenberg, G. Thiele, C. Lubbert, K.-D. Kloeppel, D. Theiss, H. Lehrach, and J. Gobom. Sample Preparation Protocols for MALDI-MS of Peptides and Oligonucleotides Using Prestructured Sample Supports. Int. J. Mass Spectrom., 226(2003) 163-180. 

In our laboratories, we have abandoned using RIA for the measurement of neuroactive steroids except where the RIA procedure has been strictly validated by prior MS identification for each experimental protocol. Immunoassays that measure low concentrations of steroids in complex biological matrices are... 

Protocol 2.11 Automated desalting protocol for ESI-MS analysis of intact proteins... 

Lochnit, G. and Geyer, R., An optimized protocol for nano-LC-M ALDI-TOE-MS coupling for the analysis of proteolytic digests of glycoproteins. Biomedical Chromatography 18(10), 841-848, 2004. 

This work had as main objectives to develop a protocol for the quantification of betulinic acid present in the leaves of Eugenia florida by using the technique GC/MS, GC/HD and HPLC/ DAD and through studies demonstrates the potential of this seasonal vegetable production as a source of natural metabolite. 

The main prerequisite for the quantification of low-molecular weight compounds by MALDl MS is the use of internal standards and optimized analysis protocols. For example, the quantification of alanine using 1- C-alanine as labeled internal standard in the system [C4CiIm][PFg]/CCA was possible with acceptable precision [14]. The method is therefore suited for screening processes, for example, for the comparison of enzyme activities in ILs. The detectability of peptides and proteins itself in this context also allows for an... 

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