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Monosaccharides high-performance liquid chromatography

Peelen, G. O. H., de Jong, J. G. N., and Wevers, R. A., High-performance liquid chromatography of monosaccharides and oligosaccharides in a complex biological matrix, Anal. Biochem., 198, 334, 1991. [Pg.281]

Tomiya, N., Suzuki, T., Awaya, J., Mizuno, K., Matsubara, A., Nakano, K., and Kurono, M., Determination of monosaccharides and sugar alcohols in tissues from diabetic rats by high-performance liquid chromatography with pulsed amperometric detection, Anal. Biochem., 206, 98, 1992. [Pg.282]

Fig. 4.3. High performance liquid chromatography (HPLC) of the monosaccharides obtained from a partially purified preparation of microbubble glycopeptide surfactant from forest soil. Following hydrolysis (in 2 N HC1 for 6 hr at 100°C) and filtration, the carbohydrate mixture was charged on a Bio-Rad HPX-87 cation exchange column. For comparison, part A shows the chromatogram (using the same HPLC column) of a standard solution, which contained 4 pg of each of three different monosaccharides (i.e., the last three peaks shown are glucose, xylose and fiicose, in the order of increasing retention times). Part B shows the chromatogram obtained from hydrolysis of the partially purified (see text) microbubble surfactant (approximately 30 pg). All other experimental conditions were identical in the two cases, i.e., water eluent, 0.5 ml/min flow rate, 85°C, refractive index detector attenuation -2x. (Taken from ref. 322.)... Fig. 4.3. High performance liquid chromatography (HPLC) of the monosaccharides obtained from a partially purified preparation of microbubble glycopeptide surfactant from forest soil. Following hydrolysis (in 2 N HC1 for 6 hr at 100°C) and filtration, the carbohydrate mixture was charged on a Bio-Rad HPX-87 cation exchange column. For comparison, part A shows the chromatogram (using the same HPLC column) of a standard solution, which contained 4 pg of each of three different monosaccharides (i.e., the last three peaks shown are glucose, xylose and fiicose, in the order of increasing retention times). Part B shows the chromatogram obtained from hydrolysis of the partially purified (see text) microbubble surfactant (approximately 30 pg). All other experimental conditions were identical in the two cases, i.e., water eluent, 0.5 ml/min flow rate, 85°C, refractive index detector attenuation -2x. (Taken from ref. 322.)...
Due to high water solubility of monosaccharides, the use of the most routine high-performance liquid chromatography (HPLC) reversed-phase columns is also not suitable for their analysis. Extremely pure solvents have to be used if ultraviolet (UV) detection is applied. If a refractometer is used as the detector (RD), extremely steady chromatographic conditions are necessary. Nevertheless, HPLC is applied in the practice. The modern approach involves the use of propylamino columns (e.g.. Refs. 3 and 4). [Pg.310]

The monomer constituents of biomacromolecules can be separated and identified by various chromatographic methods, which have been employed extensively in biochemical analyses. High performance liquid chromatography (HPLC) is a popular technique for the analysis of small biomolecules (Lim, 1986). An application of HPLC to analyze nucleotides, a-amino acids and monosaccharides is shown in Table 2.5. [Pg.28]

Wang, W., et al. (1990). High-performance Liquid Chromatography of Sialic Acid-containing Oligosaccharides and Acidic Monosaccharides, . dna/. Biochem. 190 182—187. [Pg.221]

Anumula KR (1994) Quantitative determination of monosaccharides in glycoproteins by high-performance liquid chromatography with highly sensitive fluorescence detection. Analytical Biochemistry 220 275-283. [Pg.432]

Kwon H and Kim J (1993) Determination of monosaccharides in glycoproteins by reverse-phase high-performance liquid chromatography. Analytical Biochemistry 215 243-252. [Pg.432]

J. K. Lin and S. S. Wu Synthesis of dabsylhidrazine and its use in the chromatographic determination of monosaccharides by thin-layer and high-performance liquid chromatography.Ana/. Chem. 59 1320-1326... [Pg.504]

The monosaccharide composition of the oligosaccharide or polysaccharide is determined by first hydrolyzing the sample with 10% (v/v) concentrated hydrochloric acid or trifluoroacetic acid at 100-120°C in a sealed ampule for 30-240 min. The monosaccharide composition is then analyzed by TLC [5] (see Fig. 12.1). Some laboratories might use high-performance liquid chromatography (HPLC) [8], although analysis by TLC uses much less sample and much simpler equip-... [Pg.346]

Dye C, Yttri KE (2005) Determination of monosaccharide anhydrides in atmospheric aerosols by use of high-performance liquid chromatography combined with high-resolution mass spectrometry. Anal Chem 77 1853-1858... [Pg.104]

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Monosaccharides chromatography

Monosaccharides liquid chromatography

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