Molybdenum cofactor synthesis

Molybdenum, tris(phenylenedithio)-structure, 1,63 Molybdenum alkoxides physical properties, 2,346 synthesis, 2,339 Molybdenum blue liquid-liquid extraction, 1,548 Molybdenum cofactor, 6,657 Molybdenum complexes acrylonitrile, 2,263 alkoxides, 3,1307 alkoxy carbonyl reactions, 2,355 alkyl, 3,1307 alkyl alkoxy reactions, 2,358 alkyl peroxides oxidation catalyses, 6,342 allyl, 3,1306... 

An organism such as E. coli has at least five molybdoenzymes. It will be of great interest to look at the synthesis, availability and cellular distribution of the molybdenum cofactor and its relationship to the function of these molybdoenzymes at different stages of the cell cycle. The study of chlorate-resistant1057 and nitrate reductase-deficient1058 mutants of E. coli, which are... 

The conversion of dinitrogen to ammonia is one of the important processes of chemistry. Whereas the technical ammonia synthesis requires high temperature and pressure (1), this reaction proceeds at room temperature and ambient pressure in nature, mediated by the enzyme nitrogenase (2). There is evidence that N2 is bound and reduced at the iron-molybdenum cofactor (FeMoco), a unique Fe/Mo/S cluster present in the MoFe protein of nitrogenase. Although detailed structural information on nitrogenase has been available for some time (3), the mechanism of N2 reduction by this enzyme is still unclear at the molecular level. Nevertheless, it is possible to bind and reduce dinitrogen at simple mono- and binuclear transition-metal systems which allow to obtain mechanistic information on elemental steps involved... 

The Taylor route is also very useful in the synthesis of more complex structures as demonstrated in the total synthesis of deoxyurothione (381), ( )-urothion (382) <89JA285>, and analogous 6,7-dihydrothieno[3,2- ]pterins (384) which are model substances of the oxidative breakdown product (383), termed form B of the molybdenum cofactor (399) <88JOC5839>. 

The modeling and ultimate total synthesis of molybdopterin and Mo-co have been hampered by difficulties associated with the pterin chemistry involved. However, synthetic assaults on molybdopterin, the molybdenum cofactor, and the degradation products of the molybdenum cofactor are now well underway. The total chemical synthesis of urothi-one (7), the postulated metabolic excretory product of Mo-co (25), has recently been reported (4 7). Compound 7 is a naturally occurring substituted thiophene that is found in the urine of normal humans. It is absent (25) from the urine of children who lack the molybdenum cofactor due to a genetic defect and who are unable to metabolize sulfite (48). The absolute configuration of Form A (8), another degradation product of... 

In 2010, a baby girl was diagnosed with molybdenum cofactor deficiency at 6 days old. After treatment with purified cyclic pyranopterin monophosphate (cPMP), the precursor of MoCo whose synthesis was defective, starting at 36 days old, all urinary metabolites of sulfite oxidase and xanthine oxidase deficiency returned to almost normal values and has remained constant for more than one year. 

B7.10 An organometallic approach to the synthesis of high nuclearity molybdenum-iron-sulfur clusters as potential models for the iron-molybdenum cofactor of... 

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