Molecular properties purification

Cabanes J, Escribano J, Gandia-Herrero F, Garcia-Carmona F nd Jimenez-Atienzar M. 2007. Partial purification of latent polyphenol oxidase from peach (Prumis persica L. Cv. Catherina). Molecular properties and kinetic characterization of soluble and membrane-bound forms. J Agric Food Chem 55(25) 10446-10451. 

In the earlier studies, complete purification of sialyltransferases had not been achieved, but this did not preclude the possibility of the presence of more than one sialyltransferase in an enzyme preparation, and it prevented detailed study of the molecular properties of these enzymes. This is why the results from the former studies on the specificity of substrates must be interpreted with care. Exact studies of si-... 

Kuronen, T., and Ellfolk, N. (1972). A new purification procedure and molecular properties of Pseudomonas cytochrome oxidase. Biochim. Biophys. Acta 275, 308-318. 

Bojanovski, M. Kulbe, K.D. Lamprecht, W. Liver 3-phosphoglycerate kinase. Purification and some molecular properties of the bovine-liver enzyme. Eur. J. Biochem., 45, 321-331 (1974)... 

Ben-Arie N., Khen M. and Lancet D. (1993) Glutathione S-transferase in rat olfactory epithelium purification, molecular properties and odorant biotransformation. Biochem. J. 292, 379-384. 

Initial synthesis of GMC for process development and optimization studies was accomplished on a small laboratory scale with synthetic runs typically yielding 5-15 g of polymer. However, in order to test GMC on a production basis and introduce it into manufacture, scale-up of the synthesis was necessary. The control of molecular properties and composition had to be considerably better than for most commercial polymers. To this end, a pilot plant for the manufacture of GMC was designed, constructed, and used to produce kilogram quantities of polymer. The scale-up of GMC provides an excellent example of how basic chemical engineering principles are employed in microcircuit fabrication, as well as some of the challenges in synthesis, process control, and purification. The major components of the pilot plant are shown in Fig. 6. 

Antibodies are very diverse in molecular properties, chemical characteristics, and biological activity. Purification strategies are therefore also diverse, since they are based on a large variety of molecular interactions. Antibodies have several common properties that are frequently exploited from the initial feedstock. Knowledge about the nature and concentration of impurities is the key to success. Therefore, the initial composition of feedstock is important when designing the separation process. The expression system can be selected to simplify the extraction-purification procedures. 

Hallenbeck, P. C., Meyer, C. M., and Vignais, P. M.1982. Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata purification and molecular properties. J. Bacteriol. 149, 708-717. 

A1983 review of the molecular properties of opioid receptors that includes an account of solubilization and purification procedures is available.020 ... 

NADH dehydrogenase and, 189 ubiquinone reductase and, 178,182,183 Cholesterol, side chain cleavage, 83, 84-85 Choline, oxidation to betaine, 260 Choline dehydrogenase electron transport system and, 261-263 properties, 260-201 Chromatium sulfate reduction by, 281 transhydrogenase of, 54 function of, 80 molecular properties, 58, 69 purification, 55, 56 Chromium... 

Pseudomonas aeruginosa nitrite reductase of, 274, 275 transhydrogenase of, 53 molecular properties, 57 purification, 54, 56... 

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