Molecular cell surface membrane

Figure 9.5 Schematic illustration of the experimental analysis of molecular networks of the cell surface membrane (for details see text).

Decreased epidermal proliferation is considered to be the main mechanism of action of PUVA in the treatment of psoriasis. Once excited by UVA, psoralens can react with molecular oxygen, producing reactive oxygen species that cause mitochondrial dysfunction and lead to apoptosis of skin Langerhans cells, keratinocytes, and lymphocytes [134]. PUVA further decreases epidermal cell proliferation by noncompetitively binding to epidermal growth factor receptors and directly altering the cell surface membrane. 

Markers on the cell surface or membrane of the lymphoblast can be used to classify ALL. Among the early classification system was the FAB scheme, which was based purely on morphology and cytochemistry. This system considered nuclear appearance and degree of differentiation. This is no longer used, and the current classification of acute leukemias is based on features that can be identified only by immunologic and molecular analyses.3... 

Calahan, M., Molecular properties of sodium channels in excitable membranes, in The Cell Surface and Neuronal Function (Eds C. W. Cotman, G. Poste and G. L. Nicholson), P. I, Elsevier, Amsterdam, 1980. 

Quackenbush, E., et al. Molecular doning of complementary DNAs encoding the heavy chain of the human 4F2 cell-surface antigen a type II membrane glycoprotein involved in normal and neoplastic cell growth. Proc. Natl. Acad. Sci. U. S. A. 1987, 84, 6526-6530. 

Screening the molecular heterogeneity of receptor expression in endothelial cell surfaces is required for the development of vascular-targeted therapies. First, as opposed to targeting purified proteins as discussed above, membrane-bound receptors are more likely to preserve their functional conformation, which can be lost upon purification and immobilization outside the context of intact cells. Moreover, many cell surface receptors require the cell membrane microenvironment to function so that protein-protein interaction may occur. Finally, combinatorial approaches may allow the selection of cell membrane ligands in a functional assay and without any bias about the cellular surface receptor. Therefore, even as yet unidentified receptors may be targeted. 

Turner AJ. Molecular and Cell Biology of Membrane Proteins Gly colipid Anchors of Cell Surface Proteins, Ellis Horwood, New York, 1990. 

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