Mixed lymphocyte reaction assay

The thiol group can also be found in heterocyclic compounds such as 2-mercaptobenzothiazole (9) and echinoclathrine C (10). Compound 9 was isolated from the symbiont bacterium Micrococcus sp., which was obtained from the sponge Tedania ignis [13]. The pyridine alkaloid echinoclathrine C (10) and its S-acetylated derivative, echinoclathrine B (11), were isolated from the Okinawan sponge Echinoclathria sp. [14]. The position of the hydroxyl and acylamino group on the phenyl ring in echinoclathrines (11 and 10) was recently corrected [15]. Compound 11 showed weak immunosuppressive activity in the mixed lymphocyte reaction assay with an IC50 of 9.7 pg/ml [14]. 

Table 1 Selected thieno[2,3-d]pyrimidine-2,4( 1 H,3H)-dione derivatives and their corresponding physical data. When evaluated for their effectiveness as IL-2 inhibitors using the human mixed lymphocyte reaction assay, all experimental agents had IC50 values less than 1 x 10 6M...

In 2013, Stella and coworkers made some pyrimidine compounds to evaluate the potential immunosuppressive activity in the mixed lymphocyte reaction assay. The chemistry is based on the direct amination of the tau-tomerizable heterocycles using BOP in the presence of DBU in MeCN (13BMC1209). 

The mixed lymphocyte reaction (MLR) test was performed in 96-well flat-bottomed microtiter plates. Selected experimental agents were prepared as 10 mM stock solution in DMSO and a 50-fold dilution of this was prepared in RPMI. Serial dilutions were prepared from this subsequent solution and 10 xl of the diluted stock was added to the well to give concentrations in the assay starting at 9.5 xm. In each well was placed 1.5 x 105 donor cells to give a final volume of 0.2 ml RPMI 1640 medium supplemented with 10% human serum, 2mM L-glutamine, and penicillin/streptomycin. Cells were incubated at 37°C in a humidified atmosphere containing 5% carbon dioxide for 120 hours. 3H-Thymidine (0.5 xCi) was added in the final 6 hours of incubation and cell radioactivity levels determined, which were indicative of T-cell proliferation. 

Locai iymph node assay Cytokines (iL-1, iL-1ra, TNF-a, IL-6, mixed lymphocyte reaction TGF-(5, IL-4, IL-13)... 

Products of what is now known as the HLA class II region were originally detected through the use of the mixed lymphocyte culture (MLC) assay (Bl, B3). This test uses the principle that when lymphocytes from genetically dissimilar individuals are mixed under appropriate cell culture conditions, the lymphocytes of one individual will stimulate those of the other to reproduce and form blast cells. The finding that lymphocytes from HLA-A and HLA-B identical siblings did not stimulate each other in the MLC, whereas lymphocytes from HLA-A and HLA-B identical Individuals did, suggested that the MLC reaction is related to the HLA system but is controlled by a locus separate from HLA-A and HLA-B (Y3). This... 

CELL MEDIATED CYTOTOXICITY STIMULATED IN AN ALLOGENIC MIXED LYMPHOCYTE TUMOR REACTION (MLTR-CMC) ASSAY... 

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