Microspectroscopy confocal

Kim, H.-B., Hayashi, M., Nakatani, K., Kitamura, N., Sasaki, K., Hotta, J.-I., and Masuhara, H., In situ measurements of ion exchange processes in single polymer particles laser trapping microspectroscopy and confocal fluorescence microspectroscopy, Anal. Chem., 68, 409, 1996. 

Raman microspectroscopy results from coupling of an optical microscope to a Raman spectrometer. The high spatial resolution of the confocal Raman microspectrometry allows the characterization of the structure of food sample at a micrometer scale. The principle of this imaging technique is based on specific vibration bands as markers of Raman technique, which permit the reconstruction of spectral images by surface scanning on an area. 

Piot, O., Autran, J.C., and Manfait, M. 2000. Spatial distribution of protein and phenolic constituents in wheat grain as probed by confocal raman microspectroscopy. J. Cereal Sci. 32, 57-71. 

Use of a confocal microscope (46) greatly improves Raman microspectroscopy. As shown in Fig. 3-12, confocal microscope has a pinhole (25 to 100 urn in diameter) which rejects out-of-focus signals. The smaller the pinhole size, the better the rejection. As a result, the background (substrate) signals can be... 

G. J. Puppels, Confocal Raman Microspectroscopy in Fluorescent and Luminecent Probes for Biological Activity (W. T. Mason, ed.), Life Sci. Resources Ltd. Cambridge, UK (1999). 

Fig. 8.1 Raman microspectroscopy as combination of surface-enhanced Raman spectroscopy and confocal microscopy...

Chourpa I, Douziech-Eyrolles D, Ngaboni-Okassa L, Fouquenet J-F, Cohen-Jonathan S, Souce M, Marchais H, Dubois P (2005) Molecular composition of iron oxide nanoparticles, precursors for magnetic dmg targeting, as characterized by confocal Raman microspectroscopy. Analyst 130 1395-1403... 

Labarthet, F. L., Buffeteau, X, and Sourisseau, C. Molecular orientations in azopolymer holographic diffraction gratings as studied by Raman confocal microspectroscopy, f. Phys. Chem. B 1998, 102, pp. 5754-5765. 

Figures 11.10 and 11.11 demonstrate the confocal effect for Raman microspectroscopy of polymer films (7). In Figure 11.10, the Raman intensity of a...

Puppels, G.J., De Mul, F.F.M., Otto, C., Greve, J., Robert-Nicoud, M., Arndt-Jovin, D.J. and Jovin, T.M. (1990) Studying single living cells and chromosomes by confocal Raman microspectroscopy. Nature, 347, 301-303. 

The WITec alpha300 R confocal Raman microscope can be upgraded to perform atomic force microscopy (AFM), tip-enhanced Raman spectrometry and near-field scanning optical microscopy, and is arguably the most versatile instrument for Raman microspectroscopy available today. 

E. A., Bruining, H.A. and Puppels, G.J. (2001) In vivo confocal Raman microspectroscopy of the skin noninvasive determination of molecular concentration profiles. J. Invest. 

Results from Confocal Raman Microspectroscopy of Human Cells... 

These results demonstrate that confocal Raman microspectroscopy, when coupled to image reconstruction methods such as HCA, can provide label-free methods for the visuahzation of intracellular components and processes. This subject will be elaborated further in the next section. 

Wavenumber (cm Figu re 7.2 Synchrotron infrared microspectroscopy with 6 x6pm confocal operation and truncation at low wavenumber due to diffraction showing (a) The scutellum portion of the germ with high 1 740cm lipid compared to 1650cm protein (b) The... 

While the first synchrotron IR microspectroscopy was performed at the NSLS of BNL during late 1992 [27, 28], in January 1993 the present author was able to access the temporary beamline set-up to obtain 6 x 6 pm confocal spectra of wheat cross-sections, including single cells in successive cell layers that form the bulls-eye pattern of the primary root (see Figure 7.11) [16]. The spectra in Figure 7.11... 

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