Microspectroscopic imaging

Bastiaens, P. I. and Jovin, T. M. (1996). Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein fluorescent-labeled protein kinase C beta I. Proc. Natl. Acad. Sci. USA 93, 8407-12. 

Marcott, C., Reeder, R. C., Paschalis, E. P., Talakis, D. N., Boskey, A. L. and Mendelsohn, R. (1998) Infrared microspectroscopic imaging of biomineralized tissues using a mercury-cadmium-telluride focal-plane array detector. Cell. Mol. Biol. 44, 109-115. 

Lasch, P. and Naumann, D. (1998) FT-IR microspectroscopic imaging of human carcinoma in thin sections based on pattern recognition techniques. Cell. Mol. Biol. 44(1), 189-202. 

Infrared microscopic imaging provides the significant advantages of direct spatially resolved concentration and molecular structure information for sample constituents. Raman microscopy (not further discussed in this chapter) possesses the additional benefit of confocal acquisition of this information and a 10-fold increase in spatial resolution at the expense of reduced signal-to-noise ratios compared with IR. The interested reader is urged to check the seminal studies of the Puppels group in Rotterdam,38 0 as well as our own initial efforts in this direction.41 The current section describes the initial applications of IR microspectroscopic imaging to monitor the permeation and tissue distribution of the dermal penetration enhancer, DMSO, in porcine skin as well as to track the extent of permeation of phospholipid vesicles. 

Lasch P, Diem M, Naumann D (2004) FT-IR microspectroscopic imaging of prostate tissue sections. Proc SPIE 5321 1-9... 

Steller, W., Einenkel, J., Horn, LC., Braumann, U.D., Binder, H., Salzer, R. and Krafft, C. (2006) Delimitation of squamous cell cervical carcinoma using infrared microspectroscopic imaging. Anal. Bioanal. Chem., 384, 145-54. 

Budevska, B.O., Sum, S.T. and Jones, T.J. (2003) Application of mulfivariate curve resolution for analysis of FT-IR microspectroscopic images of in situ plant tissue. Appl. Spectrosc., 57, 2. 

J. L. Koenig, Microspectroscopic Imaging of Polymers, American Chemical Society, Washington, D.C., 1998. 

Lasch P. Spectral pre-processing for biomedical vibrational spectroscopy and microspectroscopic imaging. Chemom Intell Lab Syst. 2012 117 100-14. 

Koenig JL (1998) Microspectroscopic imaging of polymers. Oxford University Press, Oxford... 

To examine small samples or small regions of larger samples, a microscope is usually required. Microspectroscopic imaging is achieved when the microscope... 

In principle, measurements with FTIR microspectroscopic imaging systems using FPA detectors are performed in the same way as with a conventional FTIR microscope [13]. Sample and background interferogram files are acquired. Fourier-transformed, and ratioed, in order to calculate a file of absorbance spectra. When the imaging system uses a FPA, each element is used to generate simultaneously... 

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