FTIR microscope

Additions to the PLM include monochromatic filters or a monochromator to obtain dispersion data (eg, the variation in refractive index with wavelength). By the middle of the twentieth century, ultraviolet and infrared radiation were used to increase the identification parameters. In 1995 the FTIR microscope gives a view of the sample and an infrared absorption pattern on selected 100-p.m areas (about 2—5-ng samples) (37). 

Figure 7.16 Schematic representation of off-line SFC-FTIR. After deposition of the eluites on to a moving ZnSe substrate the window is moved to the focus of a stand-alone FTIR microscope, where the spectmm of each spot is measured with the plate stationary. After Griffiths et al. [374]. Reprinted from P.R. Griffiths et al., in Hyphenated Techniques in Supercritical Fluid Chromatography and Extraction (K. Jinno, ed.), pp. 83-101, Copyright (1992), with permission from Elsevier...

Kempfert, K.D., Coel, B., Troost, P. and Lavery, D.S. (2001). Advancements in FTIR microscope design for faster and easier microanalysis. American Laboratory 33 (Nov) 22-27. 

To study the effects of composition on the photopolymerization behavior of acrylates, the group of Bowman et al. prepared gradient libraries where acrylate composition was varied in one dimension and light exposure was varied using a moveable shutter in the orthogonal direction [17-21]. An FTIR microscope was used to characterize the conversion across the samples and the data from multiple libraries was then used to derive kinetic models for the photopolymerization. 

Generally, this technique is used to analyze samples that are available either in small quantity or a small entity. Gels within a rubber sample, have to be microtomed (i.e., cut into very thin slices) and mounted in KBr plates in a Microscopy Lab. Samples contaminated with inorganic components are usually analyzed by both X-ray and FTIR Microscope. Sample size 20 microns can be analyzed by the FTIR-microscope. 

Camacho, N. P., West, P., Torzilli, P. A. and Mendelsohn, R. (2001) FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. Biopolymers 62, 1-8. 

Diffuse Reflectance, Attenuated Total Reflection or Multiple Internal Reflection, Photoacoustic (PAS), Photothemal Beam Deflection, Specular Reflection Absorption, and forensic applications with the diamond cell and the Fourier transform infrared (FTIR) microscope. In museum laboratories, FTIR applications have been used for problems of identification and degradation in art and archeology. (14)... 

For very small samples (in the picogram range, or with disc diameters below 0.5mm), the use of a FTIR microscope is required. A FTIR microscope is schematically shown in Fig. 4.1.5 for Bio-Rad Digilab Model UMA 300 A. The microscope permits visual inspection of the sample for selecting a spot of... 

Fig. 4.1.5. FTIR microscope UMA 300 A (Bio-Rad, Digilab) operating in the transmission / or in the reflectance 2 mode...

Griesser, U. J. and Burger, A. (1993). The polymorphic drug substances of the European Pharmacopoeia. Part 8. Thermal analytical and FTIR-microscopic investigations of etofylline crystal forms. J. Pharm. ScL, 61, 133 3. [128]... 

Most reflectance spectroscopy is carried out utilizing an accessory that can be easily inserted into and removed from the sampling compartment of a conventional spectrometer. These accessories are designed for each application and usually consist of mirrors or prisms for reflecting or focusing the radiation. Most of the sampling accessories for the FTIR spectrometers and the FTIR microscopes are available commercially. 

Kellner, R. Kiihnert-Brandstatter, M.H. Malissa, H. FTIR-microscopic investigations of microphases and microphase-transitions in organic substances. Mikrochim. Acta 1998, 2, 133-137. 

Recently Gong et al. [36] discovered that a nanostructured film grown on a microplatinum electrode also exhibits AIREs in CO adsorption. They applied a fast potential cycling treatment (between 0.0 V 1.58 V at a scan rate of 30V s ) to a micro-Pt electrode (r = 100 pm) and found that a nanostructured film ranging from a few nm to a few tens of nm in thickness was formed at the surface of Pt upon the treatment. The CO on such nanostructured film surface of micro-Pt electrode was detected by employing an in-situ FTIR microscope [37]. Similar abnormal IR... 

FTIR spectroscopy can combine with microscopy for generating FTIR spectra from microscopic volumes in materials. The instrument for FTIR microspectroscopy is simply called the FTIR microscope, which is often attached to the conventional FTIR instrument. The FTIR microscope is increasingly used for materials characterization because of its simple operation and FTIR spectra can be collected rapidly from microscopic volumes selected with the microscope. 

Figure 9.25 Optical paths of FTIR microscope with IR radiation (a) transmittance and (b) reflectance. M, mirror C, Cassegrain lens. (Reproduced by permission of PerkinElmer Inc.)...

Figure 9.26 and Figure 9.27 illustrate an example of using the FTIR microscope to identify a micro-sized particle. A contaminant particle is isolated, as shown in the micrograph (Figure 9.26). The twisted fiber is cotton. The particle attached to the fiber was examined with reflectance FTIR microspectroscopy. Figure 9.27 shows the IR spectrum of the particle and...

Raman microscopes are more commonly used for materials characterization than other Raman instruments. Raman microscopes are able to examine microscopic areas of materials by focusing the laser beam down to the micrometer level without much sample preparation as long as a surface of the sample is free from contamination. This technique should be referred to as Raman microspectroscopy because Raman microscopy is not mainly used for imaging purposes, similar to FUR microspectroscopy. An important difference between Raman micro-and FUR microspectroscopies is their spatial resolution. The spatial resolution of the Raman microscope is at least one order of magnitude higher than the FTIR microscope. 

Hyperspectrai linage mosaics of tissue sections acquired with FTIR microscope... 

Adrian Knowles (2006), FTIR microscope, httnl , http //www.jobinyvon.com/Raman/ FTIR-Microscope (accessed February 2009) Delhaye, M. and Dhamelincourt, P. (1975) J. Raman Spectrosc., 3, 33 3. 

The FTIR spectrum of a piece of paper can be measured non-destructively using an FTIR-microscope or, probably more reproducibly, by taking a sample and either placing it directly in a diamond cell or grinding it with infrared-transparent potassium bromide and pressing it into a disc to go into the spectrometer. 

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