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Microcontact-printing technique stamping

Geometrical lab chips with micropattemed surfaces are used to geometrically control the cell attachment to the matrix materials, which provide opportunities to explore the fundamental effect of geometrical-driven ceU morphology changes to stem cell differentiation. Microcontact printing techniques are widely used to pattern the shapes of individual cells on a substrate (Fig. 1). Briefly, the procedure involves the transfer of extracellular matrix proteins from the microfabricated polydimethylsiloxane (PDMS) stamp with micropattems onto the substrate. The nonpattemed... [Pg.1278]

Fig. 9.13 a) Preparation of laterally structured SAMs by the microcontact printing (pCP) technique. A structured PDMS stamp is inked with self-assembling molecules (hexa-decanethiols HDT) and placed onto a planar substrate (gold). SAM formation occurs within seconds at the areas of contact (I). The structure can be further processed by etching (II) or deposition of a second SAM (III) onto... [Pg.389]

Microcontact printing (p-CP) is another technique that can be used to place NAs onto different target surfaces. This technique makes use of an elastomeric stamp of polydimethylsiloxane (PDMS) and produces features with lateral resolution in the submicrometer range. The PDMS stamp is topographically structured by casting a PDMS prepolymer against a 3D master. The stamp is then inked with the molecules of interest, rinsed with buffer, blown dry under a stream of nitrogen, and then used to print the material onto the substrate surface (see Fig. 20). [Pg.104]

Microcontact printing (pCP) is a technique that uses an elastomeric stamp with relief on its surface to generate patterned SAMs on the surface of both planar and curved substrates [87,88]. SAMs are highly ordered molecular assemblies that form spontaneously by chemisorption of functionalized long-chain molecules on the surfaces of appropriate substrates [79,89]. Well-established systems of SAMs include alkanethiolates on coinage metals (Au, Ag, Cu) [90] alkyl-siloxanes on hydroxyl-terminated surfaces (Si/Si02, glass) [91] carboxylic and... [Pg.6]

Microcontact printing ( J,CP) [17,29,30] is part of a set of nonphotolithographic fabrication techniques known as soft lithography. In iCP, features are patterned with inexpensive, elastomeric PDMS stamps (see Section 5.5.2 for details on stamp fabrication). Consequently, [iCP can be less expensive in terms of capital and operation costs compared to photolithography for patterning large-area micron- or submicron-sized, features [31,32]. [Pg.438]

Fig. 2 Microfabrication techniques for organ-on-a-chip platforms. Use of replica molding to create PDMS (A) stamps and (B) channel with two inlets and one outlet (Adapted from [34]). (C) Microcontact printed proteins used to pattern cell (Adapted from [35]). Fig. 2 Microfabrication techniques for organ-on-a-chip platforms. Use of replica molding to create PDMS (A) stamps and (B) channel with two inlets and one outlet (Adapted from [34]). (C) Microcontact printed proteins used to pattern cell (Adapted from [35]).

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