Methylmalonic acid chromatography

G5. Gompertz, D., The measurement of urinary methylmalonic acid by a combination of thin layer and gas chromatography. Clin. Chim. Acta 19, 477-481 (1968). [Pg.209]

Indirect indicators of vitamin B12 deficiency include measurements of the metabolites homocysteine and methylmalonic acid (MMA) in serum and MMA in urine (see the Biochemical Perspectives section). Whereas the serum homocysteine concentration increases during folate or vitamin B12 deficiencies, the serum and urine MMA concentrations increase only in vitamin B12 deficiency. Therefore, MMA determinations can be used to differentiate vitamin B12 deficiency from folate deficiency. The normal concentration of MMA in serum ranges from 0.08 to 0.28 pmol/L. MMA is quantified using gas-liquid chromatography and mass spectrometry. Elevated concentrations of MMA and homocysteine in serum may precede the development of hematological abnormalities and reductions in serum vitamin B12 concentrations. One should be aware that other conditions, including renal in sufficiency and inborn errors of metabolism, can also result in elevated serum levels of MMA. [Pg.303]

Stabler SP, Marcell PD, Podell ER, Allen RH, Linden-baum J. Assay of methylmalonic acid in the serum of patients with cobalamin deficiency using capillary gas chromatography-mass spectrometry. J Clin Invest 1986 77 1606-12. [Pg.1161]

Methylmalonic aciduria Methylmalonyl-CoA isomerase or vitamin Bu coensyme Methylmalonic acid (4 glydne) Methylmalonic acid Metbylmalmiic add increased in CSF, keto-nuria, protdn intolerance Coma, extensOT spasna. retarded devdopment, metabolic addosis (vitamin Bu treats ment) Chromatography Aeetest sticks (01, R9. Rll, R12. S41. S53)... [Pg.186]

D. K. Morgan, Prederivatization Liquid Chromatography for the Determination of Inorganic Phosphate and of Methylmalonic Acid and Flow Injection Analysis with Infrared Detection. Diss. Abstr. Int. B, 46 (1986) 3421. (Ph.D. Thesis, Miami Univ., Oxford, OH, USA). [Pg.465]

The Measurement of Urinary Methylmalonic Acid by a Combination of Thin-Layer and Gas Chromatography Clin. Chim. Acta 19(3) 477-484 (1968) CA 68 66238u... [Pg.94]

Microdetermination of Methylmalonic Acid and Other Short-Chain Dicarb-oxylic Acids by Gas Chromatography Use of Prenatal Diagnosis of Methylmalonic Acidaemia and in Studies of Isovaleric Acidaemia Clin. Chim. Acta 68(2) 127-140 (1976) CA 85 1974n... [Pg.186]

Lakso HA, Appelblad P, Schneede J. Quantification of methylmalonic acid in human plasma with hydrophilic interaction liquid chromatography separation and mass spectrometric detection. Clin Chem 2008 54 2028—35. [Pg.629]

Fasching C, Singh J. Quantitation of methylmalonic acid in plasma using Uquid chromatography-tandem mass spectrometry. Meth Mol Biol 2010 603 371-8. [Pg.630]

Nakamura, E., Rosenberg, L.E. and Tanaka, K. (1976), Microdetermination of methylmalonic acid and other short chain dicarboxylic acids by gas chromatography Use in prenatal diagnosis of methylmalonic acidemia and in studies of isovaleric acidemia. Clin. Chim. Actay 68,127. [Pg.152]

Gas Chromatography-Mass Spectrometric Studies on the Urinary Organic Acids of Patients with Methylmalonic Aciduria... [Pg.143]

Chalmers, R.A., Lawson, A.M. and Watts, R.W.E. (1974b), Studies on the urinary acidic metabolites excreted by patients with )S-methylcrotonylglycinuria, propionic acidaemia and methylmalonic aciduria, using gas-liquid chromatography and mass spectrometry. Clin. Chim. Acta, 52,43. [Pg.139]

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