1 -Methyl-3-nitro-1 -nitrosoguanidine MNNG

Kim, D. J., B. W. Ahn, B. S. Han, and H. Tsuda. 1997. Potential preventive effects of Chelidonium majis L. (Papaveraceae) herb extract on glandular stomach tumor development in rats treated with V-methyl-V -nitro-V nitrosoguanidine (MNNG) and hypertonic sodium chloride. Cancer Lett. 112 203-208. 

Major sources are the basic hydrolysis of V-methyl-V-nitrosourea (or V-methyl-V-nitroso-/)-toluenesulfonamide (Diazald ) or l-methyl-3-nitro-l-nitrosoguanidine (MNNG). 

Protected amino acids or peptides are activated at the carboxy group by the mixed anhydride method using isobutyl chloroformate and NMM and reacted with ethereal diazomethane to form the corresponding peptidyl diazomethyl ketone (Scheme 1). The diazomethane solution is prepared from TV-methyl-A -nitroso-d-toluenesulfonamide (Diazald, Aldrich) or l-methyl-3-nitro-l-nitrosoguanidine (MNNG, Aldrich) according to the supplier s instruction (Aldrich information bulletin No. Al-180). No racemization occurs when diazomethyl ketones are prepared by this method. 

N-methyl-N -nitro-N-nitrosoguanidine (MNNG) stock solution (lmg/mL) in acetone (Sigma). 

Grow cells on enriched media containing the mutagen A-methyl-N, -nitro-Ar-nitrosoguanidine (MNNG) at 0.5/ig/ml or ethyl methane sulphonate (EMS) at 200/tg/ml for 16 h. 

Stoma Inhibition of /V-methyl-W-nitro-A-nitrosoguanidine (MNNG) induced gastric carinogenesis... 

Reddy and coworkers (88-90) evaluated carcinogenic or tumor-promoting activity of cholesterol, a-epoxide, triol and primary and secondary bile acids, with and without induction by N-methyl-N -nitro-N-nitrosoguanidine (MNNG). Tumor development required MNNG, and bile acids acted as promoters. Cholesterol and its metabolites were not found to have promoting activity. Other Cancers... 

A-Methyl-V -nitro-A-nitrosoguanidine (MNNG) [70-25-7] (Vol. 4, Suppl. 7 1987) (NB Overall evaluation upgraded from 2B to 2A with supporting evidence from other data relevant to the evaluation of carcinogenicity and its mechanisms)... 

Analysis of Reagent Purity diazomethane is titrated by adding a known quantity of benzoic acid to an aliquot of the solution such that the solution is colorless and excess benzoic acid remains. Water is then added, and the amount of benzoic acid remaining is back-titrated with NaOH solution. The difference between the amount of acid added and the amount remaining reveals the amount of active diazomethane present in the aliquot. Preparative Methods diazomethane is usually prepared by the decomposition of various derivatives of A-methyl-iV-nitroso-amines. Numerous methods of preparation have been described,. but the most common and most frequently employed are those which utilize N-Methyl-N-nitroso-p-toluenesulfonamide (Diazald 1), l-Methyl-3-nitro-l-nitrosoguanidine (MNNG, 2)f oriV-methyl-Mnitrosourea (3) ... 

Chemicals EDB, ethylene dibromide EMS, ethyl methanesulfonate MMS, methyl medianesul-fonate MNNG, iV-methyl-iV -nitro-N-nitrosoguanidine ICR-170, methoxy-6-chloro-9[3-(ethyl-2-chloroethyl) aminopropylamino]acridine dihydrochloride TEM, 2,4,6-tris(ethylene-imino)-iS-triazme. Table symbols (+) = positive results (—) = negative results ( ) = no tests reported. 

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