Method Development and Optimization of Enantiomeric Separations Using Macrocyclic Glycopeptide Chiral Stationary Phases

Method Development and Optimization of Enantiomeric Separations Using Macrocyclic Glycopeptide Chiral Stationary Phases 

Enantiomeric separations have become increasingly important, especially in the pharmaceutical and agricultural industries as optical isomers often possess different biological properties. The analysis and preparation of a pure enantiomer usually involves its resolution from the antipode. Among all the chiral separation techniques, HPLC has proven to be the most convenient, reproducible and widely applicable method. Most of the HPLC methods employ a chiral selector as the chiral stationary phase (CSP). 

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Beesley, T.E., Lee, J.T., and Wang, A.X., Method development and optimization of enantiomeric separations using macrocyclic glycopeptide chiral stationary phases, in Chiral Separation Techniques, Second completely revised and updated edition, Subramanian, G., Ed., Wiley-VCH Weinheim, 2001, 25. 

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