Metabolite target

Hanzlik RP, Koen YM, Theertham B, Dong Y, Fang J. The reactive metabolite target protein database (TPDB) - A web-accessible resource. BMC Bioinformatics. 2007 8 95. 

Metabolic profiling and metabolite target analysis (and metabolomics)... 

Koen, Y. M., Gogichaeva, N. V., Alterman, M. A., Hanzlik, R. P. A proteomic analysis of bromobenzene reactive metabolite targets in rat liver cytosol in vivo. Chem. Res. Toxicol. 2007, 20, 511-519. 

One of the main disadvantages of using pure culture for the production of PHB includes the high cost of the pure substrates utilized and the cost of sterile precultivation of the bacteria utilized as well as the sterile operation of the final production process. Mixed or coculture systems are widely used in several fermentation processes. There are many fermentation systems, where microorganisms assimilate one substrate and convert it to an intermediate metabolite which is converted from other microorganisms to metabolite-target product. Mixed or coculture systems will serve as an attractive addition to traditional pure culture-based technology for the production of PHB. 

Analyses focusing on a group of metabolites or watching as many metabolites as possible at specified environmental or developmental stages is called metabolite profiling . The analysis of metabolites is very complex due to the chemical diversity of these small biological molecules. On the other hand metabolite target analyses may be restricted to specific metabolites of interest, which can be selectively monitored and quantified (Weckwerth, Wenzel and Fiehn, 2004). 

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