Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Metabolite identification definitive

Indications for the formation of analogous species in microbial metabolism of LAS were found by Knepper and Kruse [33] during biotransformation of commercial LAS surfactant on an FBBR. However, the low concentrations of the tentative metabolites in the test liquor, which eluted under the applied reversed-phase (RP)-HPLC conditions somewhat earlier than the normal SPC, did not permit acquisition of full-scan mass spectra as was needed for unequivocal identification. Further evidence for the formation of the intermediate with a double bond in the alkanoate moiety was reported by Bird [103]. During biodegradation of Cn-LAS by a bacterial strain, a new UV adsorption band centred near 260 nm was detected, which was assumed to result from a double bond, although a definite confirmation could not be provided. [Pg.573]

However, the temptation to link glutathione (GSH) adducts to MBI must be avoided since the identification of metabolite adducts with GSH does not confirm MBI, albeit it may be an indicator of other adverse drug reactions (Walgren et al., 2005). By definition, exogenous nucleophiles such as GSH should offer no protection from MBI [161], and in principle, even when GSH adducts are formed from the same chemical entity responsible for MBI, the exogenous adducts themselves may not be related to the enzyme-inactivating species [174]. [Pg.221]

Of all the systems which have been utilized for the analysis of pesticide residues, combined gas chromatography-mass spectrometry afiFords a particularly useful approach because positive identification of the components of a mixture can be made without prior separation at sensitivities compatible with the limited quantities of residues generally available. From the results of recent studies involving the application of this technique, it has been demonstrated that available residue analytical methods provide efficient isolation and adequate cleanup of extracts of human, animal, and environmental media in most cases to permit gas chromatographic-mass spectrometric analyses with maximum confidence. Additionally, it has been shown that this combined technique will conveniently provide definitive and conclusive confirmation of residue identity as well as characterization of residues and their metabolites of unknown structure. [Pg.147]

Isotope Labeling. Heavy isotope labels, alone, would very likely not produce better results than radioisotope labels. Problems of isolation and purification would be more difficult than radioisotope studies due to the nature of the detection system which would be needed, mass spectrometry. However, the combined use of heavy isotope and radioisotope labeling can provide very definitive information on confirming the identification of suspected metabolites in the same manner as using dual radioisotopes, such as and H, or and How-... [Pg.315]

Holman SW, Wright P, Langley GJ. High-throughput approaches towards the definitive identification of pharmaceutical drug metabolites. 2. An example of how unexpected dissociation behaviour could preclude correct assignment of sites of metabolism. Rapid Commun Mass Spectrom 2009 23 2017-2025. [Pg.443]

Fujisawa, T. Ichise-Shibuya, K. Katagi, T. Ruzo, L. O. Takimoto, Y. Metabolism of Fungicide Diethofencarb in Gnqje (Vitis vinifera L.) Definitive Identification of Thiolactic Acid Conjugated Metabolites. J. Agric. Food Chem. 2003,51, 5329-5336. [Pg.215]


See other pages where Metabolite identification definitive is mentioned: [Pg.2263]    [Pg.130]    [Pg.130]    [Pg.157]    [Pg.215]    [Pg.352]    [Pg.76]    [Pg.490]    [Pg.251]    [Pg.200]    [Pg.328]    [Pg.198]    [Pg.329]    [Pg.232]    [Pg.67]    [Pg.47]    [Pg.69]    [Pg.132]    [Pg.1289]    [Pg.355]    [Pg.150]    [Pg.153]    [Pg.127]    [Pg.575]    [Pg.433]    [Pg.37]    [Pg.299]    [Pg.70]    [Pg.207]    [Pg.395]    [Pg.426]   
See also in sourсe #XX -- [ Pg.2263 ]




SEARCH



Definitive identification

Identification, definition

Metabolite identification

© 2024 chempedia.info