High-Throughput Approach

Griffin, T. J. Han, D. K. Gygi, S. R Rist, B. Lee, H. Aebersold, R. Parker, K. C. Toward a high-throughput approach to quantitative proteomic analysis Expression-dependent protein identification by mass spectrometry. J. Am. Soc. Mass. Spectrom. 2001,12,1238-1246. 

Guignard, L., Ozawa, K., Pursglove, S.E. et al. (2002) NMR analysis of in vi/ro-synthesized proteins without purification a high-throughput approach. FEBS Letters, 524 (1-3), 159-162. 

Of course, as the generation of biological information has moved towards high-throughput approaches, ADME/PK has also needed to aspire to similar expectations. This has led to significant automation and simplification of the ADME... 

Ramos L. et al., 2000. High-throughput approaches to the quantitative analysis of ketoconazole, apoten inhibitor of cytochrome P450 3A4, in huma plasma. Rapid Commun Mass Spectrom 14 2282. 

High-Throughput Approaches to Automated Sample Preparation.322... 

It is clear that the high-throughput approach is only possible if there are sufficient catalysts available. Fortunately, in hydrogenation it is usually possible to form the pre-catalyst simply by mixing a metal precursor and the ligand in a... 

In an extension to the studies mentioned above, the actions of 11 commercial pyrethroids on calcium influx and glutamate release were assessed using a high-throughput approach with rat brain synaptosomes [75, 76]. Concentration-dependent response curves for each commercial pyrethroid were determined and the data used in a cluster analysis. Previously characterized Type II pyrethroids that induce the CS-syndrome symptoms (cypermethrin, deltamethrin, and esfenvalerate) increased calcium influx and glutamate release, and clustered with two other ot-cyano pyrethroids (p-cyfluthrin and A-cyhalothrin) that shared these same actions. Previously characterized Type I pyrethroids (bioallethrin, cismethrin, and fenpropathrin) did not share these actions and clustered with two other non-cyano pyrethroids (tefluthrin and bifenthrin) that likewise did not elicit these actions. 

A high-throughput approach to HPLC-MS/MS for metabolite identify-cation was also described by Dear and co-workers [29], where up to six hydroxylated isomers were chromatographically resolved in 1 min with the overall cycle time reduced to 5 min on a monolithic column (4 mL/min). 

Tarbit, M.H. and Berman, High-throughput approaches for evaluating absorption, distribution, metabolism and excretion properties of lead compounds, Curr. Opin. Chem. Biol., 2, 411, 1998. 

Inoue K, Higuchi T, Okada F, Iguchi H, Yoshimura Y, Sato A, Nakazawa H (2003) The validation of column-switching LC/MS as a high-throughput approach for direct analysis pf di(2-ethylhexyl) phthalate released from PVC medical devices in intravenous solution. J Pharm Biomed Anal 31 1145-1152... 

The in situ preparation of a ruthenium-alkylidene catalyst for olefin metathesis is the first step for extending this high-throughput approach toward other catalytic transformations and opens up the way to the screening of azolium salt libraries for olefin metathesis reactions. ... 

Schmatloch S, van den Berg AMJ, Fijten MWM, Schubert US (2004) A high-throughput approach towards tailor-made water-soluble metallo-supramolecular polymers. Macromol Rapid Commun 25 321-325... 

NasruUah Ml, Webster DC (2006) Polymerization of styrene and 1-butyl acrylate by atom-transfer radical polymerization - high throughput approach. Polymer Prepr 47 217-218... 

In this chapter, we will focus on the use of CLP techniques for the synthesis of systematic copolymer libraries using high-throughput approaches. Prior to that, automated parallel optimization reactions that have been performed for different CLP techniques will be discussed. At the end of this chapter there will be a highlight on the latest synthetic approaches to synthesize well-defined polymer libraries. 

Zhang HQ, Hoogenboom R, Meier MAR et al. (2005) Combinatorial and high-throughput approaches in polymer science. Meas Sci Technol 16 203-211... 

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