Metabolic control models comparisons

Before and during stress were collected A(230 p ), A(800 Pi) and electron transport rate, J. J was taken as 02 evolution rate x 4 in 8% C02. We extimate a potential assimilation rate from J and NADPH limitation at pi of 800 ubar by modelling a potential photosynthetic flux as Am =J(Pi-r ) / (4pj +8r ) - Rd, where eventually r and Rd can be evaluated by gas exchange on the same apparatus (6). Within these constraints we can compare Am values scaled to the Am of control and this should give the metabolic effect of the stress. The same comparison between C02 gas exchange actual rates includes diffusional heterogeneity and one can quantitate its relative effect. 

The control of surface functionality by proper selection of the composition of the LB films and/or the self-assembling (amphiphatic) molecular systems can mimic many functions of a biologically active membrane. An informative comparison is that between inverted erythrocyte ghosts (Dinno et al., 1991 Matthews et al., 1993) and their synthetic mimics when environmental stresses are imposed on both systems. These model systems can assist in mechanistic studies to understand the functional alterations that result from ultrasound, EM fields, and UV radiation. The behavior of carrier molecules and receptor site functionality must be mimicked properly along with simulating disturbances in the proton motive force (PMF) of viable cells. Use of ion/electron transport ionomers in membrane-catalyst preparations is beneficial for programs such as electro-enzymatic synthesis and metabolic pathway emulation (Fisher et al., 2000 Chen et al., 2004). Development of new membranes used in artificial organs and advances in micelle reaction systems have resulted from these efforts. 

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