Metabolic biological mass spectrometry

Millington, D.S., Chace, D.H., Hillman, S.L., Kodo, N. Terada, N. (1994) In Biological mass spectrometry present and future 559-579 Diagnosis of Metabolic disease (Matsuo, T., Caprioli, R.M., Gross, M.L. Sevama. Y. eds.) John Wiley Sons. 

Kuksis, A., Myher, J. J., Yang, L.-Y. and Steiner, G. (1994) Glycerolipid metabolism with deuterated tracers, in Biological Mass Spectrometry Present and Future (eds T. Matsuo, R. M. Caprioli, M. L. Gross and Y. Sayama), John Wiley, Chichester, Sussex pp. 481-93. 

ViLMOS Kertesz Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA Walter A. Korfmacher Department of Drug Metabolism and Pharmacokinetics, Merck Research Laboratories, Kenilworth, NJ, USA Jane-Marie Kowalski Bruker Daltonics, Inc., Billerica, MA, USA Paul J. Kowalski Bruker Daltonics, Inc., Billerica, MA, USA Kristen S. Kulp Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA, USA... 

Mamer OA (1994) Metabolic profiling a dilemma for mass spectrometry. Biological Mass Spectrometry, 23 535-539. 

Schanzer, W., and Donike, M. (1992) Metabolism of boldenone in man Gas chromatographic/mass spectrometric identification of urinary excreted metabolites and determination of excretion rates. Biological Mass Spectrometry, 21,3-16. 

Benzo[b]thiophene-2,3-quinone, 5-chloro-oxidation, 4, 824 Benzothiophenes, 4, 863-934 biological activity, 4, 911-913 intramolecular acylation, 4, 761 mass spectrometry, 4, 739 metabolism, 1, 242 phosphorescence, 4, 16 reactivity, 4, 741-861 spectroscopy, 4, 713-740 structure, 4, 713-740 substituents reactivity, 4, 796-839... 

An environmental protocol has been developed to assess the significance of newly discovered hazardous substances that might enter soil, water, and the food chain. Using established laboratory procedures and C-labeled 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD), gas chromatography, and mass spectrometry, we determined mobility of TCDD by soil TLC in five soils, rate and amount of plant uptake in oats and soybeans, photodecomposition rate and nature of the products, persistence in two soils at 1,10, and 100 ppm, and metabolism rate in soils. We found that TCDD is immobile in soils, not readily taken up by plants, subject to photodecomposition, persistent in soils, and slowly degraded in soils to polar metabolites. Subsequent studies revealed that the environmental contamination by TCDD is extremely small and not detectable in biological samples. 

Barbuch, R.J., Campanale, K., Hadden, C.E. et al. (2006) In vivo metabolism of [14C]ruboxistaurin in dogs, mice, and rats following oral administration and the structure determination of its metabolites by liquid chromatogra-phy/mass spectrometry and NMR spectrometry. Drug Metabolism and Disposition The Biological Fate of Chemicals, 34, 213-224. 

Very few, if any, recent biomedical publications describe the use of ion-impact mass spectrometry without the use of GC or some other separation method because most biological samples are chemically complex. The production of clean and useful El mass spectrometric signals requires the substance of interest to be very pure, and thus direct El experiments are usually confined to preliminary studies of highly purified biomolecules or to studies on the metabolism of pure materials. Two publications that describe direct El methods applicable to biochemical analysis and neuropharmaceutical studies are those of Costa et al. (1992) and Karminski-Zamola et al. (1995). 

Analytical methods exist for measuring heptachlor, heptachlor epoxide, and/or their metabolites in various tissues (including adipose tissue), blood, human milk, urine, and feces. The common method used is gas chromatography (GC) coupled with electron capture detection (ECD) followed by identification using GC/mass spectrometry (MS). Since evidence indicates that heptachlor is metabolized to heptachlor epoxide in mammals, exposure to heptachlor is usually measured by determining levels of heptachlor epoxide in biological media. A summary of the detection methods used for various biological media is presented in Table 6-1. 

Damsten, M.C., Commandeur, J.N.M., Fidder, A., Hulst, A.G.,Touw, D., Noort, D. and Vermeulen, N.P.E. (2007) Liquid chromatography/tandem mass spectrometry detection of covalent binding of acetaminophen to human serum albumin. Drug Metabolism and Disposition The Biological Fate of Chemicals, 35 (8), 1408-1417. 

Nielsen, I.L.F., Nielsen, S.E., Ravn-Haren, G., and Dragsted, L.O., Detection, stability and redox effects of black currant anthocyanin glycosides in vivo positive identification by mass spectrometry, in Biologically-Active Phytochemicals in Food Analysis, Metabolism, Bioavailability and Function, Pfannhauser, W., Fenwick, G.R., Khokhar, S., Eds., Royal Society of Chemistry, Cambridge, U.K., 2001, pp. 389-393. 

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