Protein solubilizing membrane

Nielson, C. S. and Bjerrum, 0. J. 1977. Crossed immunoelectrophoresis of bovine milk fat globule membrane protein solubilized with non-ionic detergent. Biochim. Biophys. Acta 466, 496-509. 

Luche, S., Santoni, V. and Rabi I loud, T. (2003) Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249-253. 

CHAPS = A membrane protein solubilizing agent A yV-Dbnethyl-iV-(3-sulfopropyl)-3-([(3a, 5p. Ta, 12o )-3,7,12-trihydroxy-24-oxocholan-24-yl]ammoJ-l-propanammium bmer salts. 

Duquesne K, Sturgis JN (2010) Membrane protein solubilization. Methods Mol Biol 601 205-217... 

Son et al. underlined the dilSculties in deciphering signals characteristic for proteins and for lipids and therefore suggested a q-titration of long-chain and short-chain lipids that allows differentiation between structured and mobile residues of membrane proteins. The term q-titration refers to the comparison of signal intensities in solution NMR spectra of uniformly labeled membrane proteins solubilized in micelles and isotropic bicelles as a function of the molar ratios (q) of the long-chain lipids e.g. DMPC) to short-chain lipids e.g. DHPC). 

Figure 10.3(a) Hill plot of sup-20 protein with LOC treatment, c, protein concentration, mgml C, maximal = 24%. The vertical arrow (f) indicates the CMC. (b) Hill plot of erythrocyte membrane protein solubilized with NaDS treatment, c, protein concentration, mg ml L C, maximal = 100%. The ratio of n for the part of the curve above the break to that below indicates an increased level of binding above the CMC, in (a) of 3.4 times, and in (b) of 7 times. From Foster et al. [28] with permission. LOC is composed of a diethanolamide/polyoxyethylene alcohol mixture. 

Uses For use in membrane protein solubilization, enzymology and enzyme production, analytical applies, (chromatography), electrophoresis, molecular biology, antigen and vaccine preparations, drug delivery, liposomes Properties Wh, cryst, complete sol, in water m,w, 288,38 99% purity Storage Store under cover R,T,... 

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