Membrane biohybrid system

In the last few years the use of membrane biohybrid systems has contributed to giving important information about the effect of various drugs, such as diclofenac, rofecoxib, paracetamol the effects of which on the specific functions of human hepatocyte are not completely known [17-21],... 

A protocol of isolation and culture of hippocampal neurons has been optimized by using a hibernating rodent, the hamster Mesocricetus auratus as our animal model [57]. Preliminary results demonstrated the feasibility of culturing hippocampal neurons in a membrane biohybrid system. The substrate is of great importance for the survival and differentiation of neuronal cultures. As substrate we used a gas-permeable (C02, 02, and H20 vapor) fluorocarbon foil membrane (FC), modified with a coating of poly-L-lysine in order to improve the interactions with cells. 

This study reports on the potentiality of applying a membrane biohybrid system in the field of tissue engineering and regenerative medicine evidencing the crucial points in the in-vitro reconstruction of the physiological tissue model. A number of issues need to be addressed the morphological and physicochemical properties of the membrane, the optimal density of immobilized cells, the interaction of cells with the membrane, the differentiation of cells as well as the maintenance of viability and... 

This paper reports on the development of membrane systems to be used for cell culture (e.g., hepatocytes, lymphocytes, neuronal cells) in biohybrid systems such as a therapeutic device or as in-vitro model systems for studying the effects of various drugs and chemicals on cell metabolism. 

De Bartolo, L., MoreUi, S., Rende, M., Gordano, A., and Drioh, E., New modified polyetheretherketone membrane for liver cell culture in biohybrid systems Adhesion and specific functions of isolated hepatocytes. Biomaterials, 25, 3621, 2004. 

Krasteva, N., Harms, U., Albrecht, W., Seifert, B., Hopp, M., Altankov, G., and Groth, T. (2002). Membranes for biohybrid liver support systems—Investigations on hepatocyte attachment, morphology and growth. Biomaterials 23(12), 2467-2478. 

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