Matrix culture

Minami Y, Sugihara H, Oono S. Reconstruction of cornea in three-dimensional collagen matrix culture. Invest Ophthalmol Vis Sci 34 2316-2324 (1993). 

Wakabayashi, T. et al., Borrelidin is an angiogenesis inhibitor disruption of angiogenic capillary vessels in a rat aorta matrix culture model, J. Antibiot. (Tokyo), 50, 671, 1997. 

FIGURE 35.1 Schematic of a controlled 3D matrix culture system for directing stem cell differentiation. Picture denotes 3D cell culture matrix in 4-well chamber slide, (a) Fibrin (b) PEGylated fibrin. (Reproduced with permission from Zhang, G. et al. Acta Biomater, 2010.)... 

Fig. 4. Schematic of a fixed-bed process for culturing mammalian cell using ceramic matrix.

F9 embryonal carcinoma cells have a simple set of growth supplements which are required for growth in serum-free medium insulin, transferrin, and fibronectin (Rizzino and Sato, 1978). Fibronectin is a component of the extracellular matrix and facilitates the attachment of the cells to the culture dish. In addition, high density lipoprotein (HDL) has been observed to promote the growth of F9 cells serum-free. 

Tibbitt MW, Anseth KS (2009) Hydrogels as extracellular matrix mimics for 3d cell culture. Biotechnol Bioeng 103 655-663... 

May, L.A. et al.. Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry. Anal. Biochem., 337, 62, 2005. 

Extraction of rhizosphere soil (22,34,51,52) is an approach that can provide information about long-term accumulation of rhizosphere products (root exudates and microbial metabolites) in the soil. Culture systems, which separate root compartments from adjacent bulk soil compartments by steel or nylon nets (52-54) have been employed to study radial gradients of rhizosphere products in the root environment. The use of different extraction media can account for different adsorption characteristics of rhizosphere products to the soil matrix (22,34). However, even extraction with distilled water for extended periods (>10 min) may... 

Saenz, A. J. Petersen, C. E. Valentine, N. Gantt, S. L. Karman, K. H. Kingsley, M. T. Wahl, K. L. Reproducibility of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry for replicate bacterial culture analysis. Rapid Comm. Mass Spectrom. 1999,13,1585-1585. 

The focus of this chapter is the development of a technique often called wholecell matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) or whole-cell MALDI-TOF MS. Some groups prefer to use terms such as intact or unprocessed rather than whole, but the intended meaning is the same regardless of which word is used. As noted in the first chapter of this book, there are many different methods for the analysis of bacteria. However, for the analysis of intact or unprocessed bacteria, whole-cell MALDI-TOF MS is the most commonly used approach. This method is very rapid. MALDI-TOF MS analysis of whole cells takes only minutes because the samples can be analyzed directly after collection from a bacterial culture suspension. Direct MALDI MS analysis of fungi or viruses is similar in approach1,2 but is not covered in this chapter. MALDI-TOF MS of whole cells was developed with very rapid identification or differentiation of bacteria in mind. The name (whole cell) should not be taken to imply that the cells are literally intact or whole. Rather, it should be taken to mean that the cells that have not been treated or processed in any way specifically for the removal or isolation of any cellular components from any others. In whole-cell analysis the cells have been manipulated only as necessary to... 

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