Matrix-assisted laser desorption/ionization MALDI spectrometry, degradation

With the identities and amounts of amino acids known, the peptide is sequenced to find out in what order the amino acids are linked together. Much peptide sequencing is now done by mass spectrometry, using either electrospray ionization (ESI) or matrix-assisted laser desorption ionization (MALDI) linked to a time-of-flight (TOF) mass analyzer, as described in Section 12.4. Also in common use is a chemical method of peptide sequencing called the Edman degradation. [Pg.1031]

Elucidation of degradation pathways and identification of transformation products (TPs) is of crucial importance in understanding their fate in the environment and requires the employment of advanced instrumental techniques. Analytical methods that can be used for this purpose include Uquid chromatography with diode array or fluorescence detector (LC-DAD/FL), nuclear magnetic resonance (NMR), infrared spectroscopy (IR), matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), gas... [Pg.44]

Mass spectrometry This is often used for analysis of plastics. Thermal degradation can be carried out using an ion source, or in a pyrolyzer connected directly to a mass spectrometer or via a chromatographic column. Two soft ionization techniques, electrospray ionization and matrix-assisted laser desorption ionization (MALDI), are the most common in the analyses of large molecules. MALDI-MS is usually used offline but online connection with a... [Pg.3728]

PCL was also degraded to 6-hydroxyhexanoic acid during enzymatic hydrolysis by Lipase Asahi derived from Chromobacterium viscosum and Hp-ase F derived from Rhizopus niveus [74]. In another study formation of oligomers during biotic hydrolysis of PCL was shown by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) [50]. Enzymatic degradation of copolymers of 3-hydroxybutyric acid (3HB) and... [Pg.93]

Ichiki Y, Ishizawa N, Tamura H, Teramoto K, Sato H, Yoshikawa H. Environmental distribution and novel high-throughput screening of APEO-degrading bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). J Pestic Sci. 2008 33(2) 122-7. [Pg.251]