Mass spectroscopy matrix assisted laser desorption

Nuclear Magnetic Resonance Spectroscopy Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDl-TOF MS) UV-vis Absorption Properties... 

Laser desorption and ionization laser-induced breakdown spectroscopy, matrix-assisted laser desorption and ionization, and aerosol time-of-flight mass spectrometry... 

Instrumentation used for polymer oxidative stability (Chapter 11) includes, thermogravimetric analysis, differential scanning calorimetry, evolved gas analysis, infrared spectroscopy and ESR spectroscopy, matrix assisted laser desorption/ionisation mass spectrometry and imaging chemiluminescence is included. 

Mass spectrometry. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectroscopy has been used to determine fetd/fetc in S and MMA polymerization. Chains formed by disproportionation and chains formed by combination form two distinct distributions. 

The molecular weights and molecular weight distributions (MWD) of phenolic oligomers have been evaluated using gel permeation chromatography (GPC),23,24 NMR spectroscopy,25 vapor pressure osmometry (VPO),26 intrinsic viscosity,27 and more recently matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).28... 

Mass spectroscopy is a useful technique for the characterization of dendrimers because it can be used to determine relative molar mass. Also, from the fragmentation pattern, the details of the monomer assembly in the branches can be confirmed. A variety of mass spectroscopic techniques have been used for this, including electron impact, fast atom bombardment and matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. 

Considering these situations, the observation of molecular weights, particularly by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MASS), is essential [33]. The operation is simple and enables us to observe the molecular ion peaks of CPOs with molecular weights exceeding 10,000. The quahty of the measurement is strongly dependent on the choice of the matrix. Therefore, the search for the best matrix for each CPO should be pursued. 

Wang, Z. Russon, L. Li, L. Roser, D. C. Long, S. R. Investigation of spectral reproducibility in direct analysis of bacteria proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy. Rapid Comm. Mass Spectrom. 1998,12,456-464. 

Wingerath, T. et al. (1999). Analysis of cyclic and acyclic analogs of retinol, retinoic acid, and retinal by laser desorption ionization-, matrix-assisted laser desorption ionization-mass spectrometry, and UV/Vis. spectroscopy. Anal. Biochem. 272(2) 232-242. 

After device construction, structural and functional analysis are critical. One might argue that only the second issue matters, but structural data often give insights into why devices perform suboptimally, and provide important clues about how to improve device function. We routinely use protein analytics (matrix-assisted laser desorption-ionization mass spectroscopy, amino acid composition analysis, gel electrophoresis, Western blotting, circular dichroism, vari-... 

The first of two stays of the senior author at Iowa State University was supported by a contract between Ae United Nations Industrial Development Organization and Ae National Chemical Laboratory, administered in the United States by the Carl Duisberg Society, Inc. This work was also supported by the Engineering Research Institute of Iowa State University. The authors thank Ron Niece, Cynthia Wadsworth, Nick Strickland, and Tony Grabski of the University of Wisconsin Biotechnology Center for providing confirmation of the amino acid sequence, and Brian Chait and Ron Beavis of Rockefeller University for attempting matrix-assisted laser desorption mass spectroscopy on Ac enzyme. This article is N(X Communication No. 4950. 

On-line conpling of capillary electromigration techniques with nuclear magnetic resonance spectroscopy [5] and matrix-assisted laser desorption/ionization (MALDl) time-of-flight (TOF) mass spectrometry [6] has also been reported. 

Mass spectra are used to determine molecular weights and molecular composition (from the parent or molecular ion) and to obtain structural information from the fragmentation of the molecular ion into daughter ions. Electrospray ionization (ESI-MS) and matrix-assisted laser desorption ionization (MALDI-MS) mass spectroscopy can be used to obtain structural information about macromolecules, including proteins, polymers, and drug-DNA complexes. 

MALDI MCM-41 MCR MD ME MEM MI MPM MRI MS MVA Matrix-assisted Laser Desorption/Ionization Mobile Crystalline Material-41 Multivariate Curve Resolution Molecular Dynamics Matrix-enhanced Magnetic Force Micrscopy Multivariate Image Multiphoton Microscopy Magnetic Resonance Imaging Mass Spectroscopy Multivariate Analysis... 

The low volatility of ILs makes them useful as solvents working under high vacuum, and together with their more amorphous solid analogs they are convenient in matrix-assisted laser-desorption/ ionization-mass spectroscopy (MALDI-MS) analysis.103104... 

Figure 6 The SELDI technology. This type of proteomic analytical tool is a class of mass spectroscopy instrument that is useful in high-throughput proteomic fingerprinting of serum. Using a robotic sample dispenser, 1 p,L of serum is applied to the surface of a protein-binding chip. A subset of the proteins in the sample binds to the surface of the chip. The bound proteins are treated with a matrix-assisted laser desorption and ionization matrix and are washed and dried. The chip, which contains multiple patient samples, is inserted into a vacuum chamber where it is irradiated with a laser. The laser desorbs the adherent proteins and causes them to be launched as ions. The TOF of the ion before detection by an electrode is a measure of the mass-to-charge (m/z) value of the ion. The ion spectra can be analyzed by computer-assisted tools that classify a subset of the spectra by characteristic patterns of relative intensity (adapted from www.evmsdoctors.com).

Dendrimer characterization was accomplished by 13C, NMR and FT-IR spectroscopy as well as mass spectrometry using fast atom bombardment (FAB) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) techniques. Molecular ion base peaks were observed in the MALDI-TOF MS of polyester 168 (mlz 18900 (calcd. 19044)) along with minor peaks at 37000 and 54000 mlz corresponding to ionic gasphase dimer and trimer complexes. 

The two additives have heen identified hy matrix assisted laser desorption ionisation mass spectrometry (MALDI-MS) and attenuated total reflection infrared spectroscopy (ATR). 

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