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Orbitrap mass spectrometers

A. Makarov, E. Denisov, A. Kholomeev, W. Balschun, O. Lange, K. Strupat, and S. Homing. Performance Evaluation of a Hybrid Linear Ion Trap/Orbitrap Mass Spectrometer. Anal. Chem., 78(2006) 2113-2120. [Pg.85]

Capillary electrophoresis (CE) either coupled to MS or to laser-induced fluorescence (LIF) is less often used in metabolomics approaches. This method is faster than the others and needs a smaller sample size, thereby making it especially interesting for single cell analysis [215] The most sensitive mass spectrometers are the Orbitrap and Fourier transform ion cyclotron resonance (FT-ICR) MS [213]. These machines determine the mass-to-charge ratio of a metabolite so accurate that its empirical formula can be predicted, making them the techniques of choice for the identification of unknown peaks. [Pg.151]

With time of flight instruments, a mass accuracy better than 5 ppm can be achieved, while with Fourier transform ion cyclotron resonance or orbitrap mass spectrometers mass accuracies better than 1 ppm have been reported. It is obvious that, for good mass accuracies, the peaks must be baseline resolved and resolution plays an essential role. For the present example, a mass resolving power of 5000 seems to be quite acceptable. In the case of the [M+H]+ + 1 isotope peak, the situation becomes somewhat more complex for molecules containing nitrogen, sulfur or carbon. Figure 1.5 D illustrates at a mass resolving power of 500000 the contribution of... [Pg.9]

A similar approach using accurate mass measurements and predictive fragmentation sofiware was also applied for the examination of the human microsomal metabolism of nefazodone using a linear ion trap-orbitrap hybrid mass spectrometer. Based on a single LC-MS run, using data-dependant acquisition, 15 metabolites of nefazodone could be identified in MS and MS/MS with a mass accuracy better than 3 ppm. [Pg.49]

Makarov, A. Denisov, E. Kholomeev, A. Balschun, W. Lange, O. Strupat, K. Homing, S. Performance evaluation of a hybrid linear ion trap/ orbitrap mass spectrometer. Anal. Chem. 2006, 78, 2113-2120. [Pg.62]

Ruan, Q., Peterman, S., Szewc, M. A., Ma, L., Cui, D., Humphreys, W. G., and Zhu, M. (2008). An integrated method for metabolite detection and identification using a linear ion trap/Orbitrap mass spectrometer and multiple data processing techniques Application to indinavir metabolite detection. J. Mass Spectrom. 43 251-261. [Pg.79]

Figure 5.6. Schematic of a hybrid LTQ-Orbitrap mass spectrometer (a) transfer octapole (b) curved rf-only quadmpole (C-trap) (c) gate electrode (d) trap electrode (e) ion optics (f) inner orbitrap electrode (g) outer orbitrap electrodes (Makarov et at., 2006a). Figure 5.6. Schematic of a hybrid LTQ-Orbitrap mass spectrometer (a) transfer octapole (b) curved rf-only quadmpole (C-trap) (c) gate electrode (d) trap electrode (e) ion optics (f) inner orbitrap electrode (g) outer orbitrap electrodes (Makarov et at., 2006a).
The sensitivity of the LTQ-Orbitrap is demonstrated using a mixture of synthetic standards of buspirone metabolites spiked into a rat plasma extract. The samples were analyzed with a standard 4.6-mm HPLC column. LC-MS/MS chromatograms for the five-component mixture obtained using the Orbitrap and the LTQ mass spectrometers are compared in Figs 5.8a and h. Also shown are MS/MS (m/z 402) spectra of the oxa-buspirone metabolite (R, = 7.4 min) at 10 pg on column in the Orbitrap (Fig. 5.8c) and LTQ (Fig. 5.8e). As discussed above, the MS/MS fragmentation spectra, obtained in the Orbitrap (Figs 5.8c and d), are very similar to those from the LTQ (Fig. 5.8e), and even at such low concentrations excellent mass accuracies are maintained. [Pg.206]

Chen, G., Khusid, A., Daaro, I., Irish, P., and Pramanik, B. N. (2007). Structural identification of trace level enol tautomer impurity by on-line hydrogen/deuterium exchange HR-LC/MS in a LTQ-Orbitrap hybrid mass spectrometer. J. Mass Spectrom. 42 967-970. [Pg.217]

Macek, B., Waanders, L. F., Olsen, J. V., and Mann, M. (2006). Top-down protein sequencing and MS3 on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer. Mol. Cell. Proteomics 5 949-958. [Pg.218]

Makarov, A., Denisov, E., Lange, O., and Homing, S. (2006b). Dynamic range of mass accuracy in LTQ Orbitrap hybrid mass spectrometer. J. Am. Soc. Mass Spectrom. 17 977-982. [Pg.218]

McAlister, G. C., Phanstiel, D., Good, D. M., Berggren, W. T., and Coon, J. J. (2007). Implementation of electron-transfer dissociation on a hybrid linear ion trap-orbitrap mass spectrometer. Anal. Chem. 79 3525-3534. [Pg.219]

Technological advances of ion-trap mass spectrometers are the ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and the recently released technique, the Orbitrap Fourier transform mass spectrometry (Hu et al., 2005), which enable the determination of molecular formulae with a high mass resolution and mass accuracy in mixtures. Today these ion-trap mass spectrometers are most frequently coupled with atmospheric pressure ionization (API) techniques such as electrospray ionization (ESI) (e.g., Fievre et al., 1997 Qian et al., 2001 Kujawinski et al., 2002 Llewelyn et al., 2002 Stenson et al., 2002,2003 Fard et al., 2003) or matrix-assisted laser desorption/ionization (MALDI) (e.g., Solouki et al.,... [Pg.547]

An entirely new principle of the ion-trap mass spectrometers is the Orbitrap with a coaxial inner spindle-shaped electrode in an outer barrel-like electrode. The peripheral injected ions move due to their electrostatic attraction to the inner electrode on orbits around and swing simultaneously along the electrode. The frequency of these harmonic oscillations is inversely proportional to the square root of mJz. The detected signals are induced by the frequency of these swings and resulted... [Pg.548]

Accordingly, Gordon et al. used synthetic reference compounds for metabolites of bemesetron (MDL 72,222) to evidence proposed structures (Table 7) [37], However, in contrast to the IT and SQ analysers Chen et al., He et al. and Gordon et al. used, more modem mass spectrometers providing high-resolution equipment, e.g. Orbitrap, FT-ICR or double time-of-flight (TOF-TOF), would allow more adequate and precise identification by determination of the accurate mass. [Pg.337]


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See also in sourсe #XX -- [ Pg.205 , Pg.209 , Pg.212 , Pg.213 , Pg.216 ]

See also in sourсe #XX -- [ Pg.473 ]




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